Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1231958..1232637 Replicon chromosome
Accession NZ_CP049852
Organism Escherichia coli strain UPEC-774U/190308

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag G9W34_RS05850 Protein ID WP_000057523.1
Coordinates 1231958..1232260 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag G9W34_RS05855 Protein ID WP_000806442.1
Coordinates 1232296..1232637 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9W34_RS05830 (1227221) 1227221..1228441 - 1221 WP_001773866.1 fosmidomycin MFS transporter -
G9W34_RS05835 (1228659) 1228659..1230311 + 1653 WP_001773867.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
G9W34_RS05840 (1230348) 1230348..1230827 - 480 WP_001538397.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
G9W34_RS05845 (1231031) 1231031..1231825 - 795 WP_001538398.1 TraB/GumN family protein -
G9W34_RS05850 (1231958) 1231958..1232260 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
G9W34_RS05855 (1232296) 1232296..1232637 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
G9W34_RS05860 (1232695) 1232695..1235199 - 2505 WP_000083948.1 copper-exporting P-type ATPase CopA -
G9W34_RS05865 (1235461) 1235461..1236393 + 933 WP_001538399.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T151718 WP_000057523.1 NZ_CP049852:1231958-1232260 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T151718 NZ_CP065687:c592140-591784 [Burkholderia humptydooensis]
ATGAAAATCAGTGAACTGGCCGAGTTCGACGGCTCGAAGTACCTGAAGGACGAAGAAACAATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAATCCGCGCCTGATTCAAGCCGCGCTTGGGAACGTTGCGAAGGCGCGCGGCATGACGGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTACCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCGACGATCATG
AAAGTTGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT151718 WP_000806442.1 NZ_CP049852:1232296-1232637 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT151718 NZ_CP065687:c592439-592143 [Burkholderia humptydooensis]
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTCGACGGGCTTCGCGACCCGGTCGGTAGCGCGGCGATCAA
CCTGCGCATCGAGCGGGCAAAGCTTGGCAATCTCGGCCAATGGCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTACTTCGTGCGACGCGGAAAAATTATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCCGGCGAACTGGAGGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References