Detailed information of TA system TA151709

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 613002..613414
• Replicon: chromosome
• Accession: NZ_CP049852
• Organism: Escherichia coli strain UPEC-774U/190308

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: G9W34_RS02945
• Protein ID: WP_000132614.1
• Coordinates: 613002..613343 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	613338..613414 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9W34_RS02925 (608071)	608071..609351	-	1281	WP_001338077.1	DUF445 domain-containing protein	-
G9W34_RS02930 (609536)	609536..610456	+	921	WP_001513532.1	Rpn family recombination-promoting nuclease/putative transposase	-
G9W34_RS02935 (610684)	610684..610764	+	81	WP_020233658.1	hypothetical protein	-
G9W34_RS24880 (610862)	610862..611738	+	877	Protein_573	DUF262 domain-containing protein	-
G9W34_RS24885 (611792)	611792..612955	+	1164	WP_224153787.1	DUF1524 domain-containing protein	-
G9W34_RS02945 (613002)	613002..613343	-	342	WP_000132614.1	endoribonuclease SymE	Toxin
- (613338)	613338..613414	+	77	NuclAT_8	-	Antitoxin
- (613338)	613338..613414	+	77	NuclAT_8	-	Antitoxin
- (613338)	613338..613414	+	77	NuclAT_8	-	Antitoxin
- (613338)	613338..613414	+	77	NuclAT_8	-	Antitoxin
- (613338)	613338..613414	+	77	NuclAT_9	-	Antitoxin
- (613338)	613338..613414	+	77	NuclAT_9	-	Antitoxin
- (613338)	613338..613414	+	77	NuclAT_9	-	Antitoxin
- (613338)	613338..613414	+	77	NuclAT_9	-	Antitoxin
G9W34_RS02950 (613564)	613564..615333	-	1770	WP_001513535.1	restriction endonuclease subunit S	-
G9W34_RS02955 (615333)	615333..616802	-	1470	WP_001387312.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T151709 WP_000132614.1 NZ_CP049852:c613343-613002 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T151709 NZ_CP065682:2355229-2355510 [Brevibacterium casei]
GTGATCAGATCGTTCGGGAGCAAGGAGACCGAACGGCTCTGGCGTCGTGAGCGCGTGCGGTCGATCGATCCGAGGATTCA
TCGGGTCGCATTGCGCAAGCTTCGTCAGGTGGGCTCAGCTGAGGTGCTCGAGGACCTCAGTGTTCCGCCGGGCAACCGGC
TCGAAGCGCTCAAGGGTGATCGCGCCGGGCAGCACAGCATCAGGATCAACGACCAGTGGCGGATCTGTTTCGTATGGACG
GACGCCGGACCAGAGGAGGTGGAGATCGTTGACTACCACTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT151709 NZ_CP049852:613338-613414 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin