Detailed information of TA system TA151704

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 67390..67643
• Replicon: plasmid pEcoUTI1d
• Accession: NZ_CP049848
• Organism: Escherichia coli strain UPEC-245U/190328

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: G9W35_RS26005
• Protein ID: WP_001336447.1
• Coordinates: 67494..67643 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	67390..67446 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9W35_RS25975 (63348)	63348..64094	+	747	WP_032277634.1	conjugal transfer pilus acetylase TraX	-
G9W35_RS25980 (64149)	64149..64706	+	558	WP_000139329.1	fertility inhibition protein FinO	-
G9W35_RS25985 (64858)	64858..65061	+	204	WP_001336517.1	hypothetical protein	-
G9W35_RS25990 (65803)	65803..66264	+	462	WP_064772783.1	thermonuclease family protein	-
G9W35_RS25995 (66367)	66367..66705	+	339	WP_064772784.1	hypothetical protein	-
G9W35_RS26000 (66858)	66858..67295	+	438	WP_000872609.1	hypothetical protein	-
- (67390)	67390..67446	-	57	NuclAT_1	-	Antitoxin
- (67390)	67390..67446	-	57	NuclAT_1	-	Antitoxin
- (67390)	67390..67446	-	57	NuclAT_1	-	Antitoxin
- (67390)	67390..67446	-	57	NuclAT_1	-	Antitoxin
G9W35_RS26005 (67494)	67494..67643	+	150	WP_001336447.1	Hok/Gef family protein	Toxin
G9W35_RS26010 (67928)	67928..68188	+	261	WP_000078109.1	replication regulatory protein RepA	-
G9W35_RS26015 (68292)	68292..68476	+	185	Protein_89	protein CopA/IncA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..68488	68488

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T151704 WP_001336447.1 NZ_CP049848:67494-67643 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T151704 NZ_CP065673:c3000408-3000130 [Serratia plymuthica]
GTGACACTCAAGTGGACGGAACCCGCAATTGCCGATCGTATGGCAATTTATGACTACCTGGAAACAAAGAATCCGGCCGC
AGCGATAGAAATTGATAGCTTGATTTCAGCAACTGCGACACGACTCTCACAAAACCCGTCCAGTGGAAAACCAGGCCGAG
TACCCGGTACACGTGAAATGGTTATTCATCATTCCTATTTCCTGGTTTATGAATTAATCAACGGGTTAATCATTATGCTG
GCCGTAGTGCATGCCCGCCGTCAGTGGCCCGCCAATTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT151704 NZ_CP049848:c67446-67390 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin