Detailed information of TA system TA151677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 796169..796771
• Replicon: chromosome
• Accession: NZ_CP049845
• Organism: Escherichia coli strain UPEC-245U/190328

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: G9W35_RS03805
• Protein ID: WP_000897305.1
• Coordinates: 796460..796771 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G9W35_RS03800
• Protein ID: WP_000356397.1
• Coordinates: 796169..796459 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9W35_RS03775 (792096)	792096..792998	+	903	WP_074499718.1	formate dehydrogenase O subunit beta	-
G9W35_RS03780 (792995)	792995..793630	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
G9W35_RS03785 (793627)	793627..794556	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
G9W35_RS03790 (794886)	794886..795128	-	243	WP_001086388.1	protein YiiF	-
G9W35_RS03795 (795346)	795346..795564	-	219	WP_001297075.1	CopG family transcriptional regulator	-
G9W35_RS03800 (796169)	796169..796459	-	291	WP_000356397.1	NadS family protein	Antitoxin
G9W35_RS03805 (796460)	796460..796771	-	312	WP_000897305.1	hypothetical protein	Toxin
G9W35_RS03810 (797000)	797000..797908	+	909	WP_001162704.1	alpha/beta hydrolase	-
G9W35_RS03815 (797972)	797972..798913	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
G9W35_RS03820 (798958)	798958..799395	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
G9W35_RS03825 (799392)	799392..800264	-	873	WP_000920762.1	virulence factor BrkB family protein	-
G9W35_RS03830 (800258)	800258..800857	-	600	WP_001295269.1	glucose-1-phosphatase	-
G9W35_RS03835 (800956)	800956..801741	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T151677 WP_000897305.1 NZ_CP049845:c796771-796460 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T151677 NZ_CP065656:465218-465580 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT151677 WP_000356397.1 NZ_CP049845:c796459-796169 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT151677 NZ_CP065656:465051-465221 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTCCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7