Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2273723..2274285 Replicon chromosome
Accession NZ_CP049839
Organism Escherichia coli strain UPEC-455U/190220

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag G9W36_RS11115 Protein ID WP_000605675.1
Coordinates 2274007..2274285 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag G9W36_RS11110 Protein ID WP_000781370.1
Coordinates 2273723..2274007 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9W36_RS11095 (2268904) 2268904..2271951 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
G9W36_RS11100 (2271964) 2271964..2272848 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
G9W36_RS11105 (2272841) 2272841..2273494 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
G9W36_RS11110 (2273723) 2273723..2274007 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
G9W36_RS11115 (2274007) 2274007..2274285 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
G9W36_RS11120 (2274471) 2274471..2275481 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
G9W36_RS11125 (2275615) 2275615..2277312 - 1698 WP_000433460.1 malate dehydrogenase -
G9W36_RS11130 (2277469) 2277469..2277606 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
G9W36_RS11135 (2277708) 2277708..2277923 - 216 WP_000495766.1 biofilm-dependent modulation protein -
G9W36_RS11140 (2278268) 2278268..2278699 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T151652 WP_000605675.1 NZ_CP049839:c2274285-2274007 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T151652 NZ_CP065630:427164-427445 [Brevibacterium casei]
GTGATCAGATCGTTCGGGAGCAAGGAGACCGAACGGCTCTGGCGTCGTGAGCGCGTGCGGTCGATCGATCCGAGGATTCA
TCGGGTCGCATTGCGCAAGCTTCGTCAGGTGGGCTCAGCTGAGGTGCTCGAGGACCTCAGCGTTCCGCCGGGCAACCGGC
TCGAAGCGCTCAAGGGTGATCGCGCCGGGCAGCACAGCATCAGGATCAACGACCAGTGGCGGATCTGTTTCGTATGGACG
GACGCCGGACCAGAGGAGGTGGAGATCGTTGACTACCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT151652 WP_000781370.1 NZ_CP049839:c2274007-2273723 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT151652 NZ_CP065630:427432-427734 [Brevibacterium casei]
TTGACTACCACTGACAAGATCGAACCGATCCACCCAGGTGAGGTCCTCATGGAGGACTTCATCGAAGGGTTCGGGATCAC
CCAGAATAAGCTCGCCGTGTCGATCGGAGTGCCGCCGCGCCGGATCAACGAGATCGTGCACGGCAAGCGTGGGATCACGG
CGGATACCGCATTGCGGCTCGCGAAGTATTTCGGGACGTCTCCGGAGTTCTGGATCAATCTGCAGAGCCACTACGAATTG
GATCGTGCGGAGGACGCCGCGGGCGAGCAAATCGCGGCGATCACCCCGCTGCGGGTTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References