Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1232328..1233007 Replicon chromosome
Accession NZ_CP049839
Organism Escherichia coli strain UPEC-455U/190220

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag G9W36_RS05850 Protein ID WP_000057523.1
Coordinates 1232328..1232630 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag G9W36_RS05855 Protein ID WP_000806442.1
Coordinates 1232666..1233007 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9W36_RS05830 (1227591) 1227591..1228811 - 1221 WP_001773866.1 fosmidomycin MFS transporter -
G9W36_RS05835 (1229029) 1229029..1230681 + 1653 WP_001773867.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
G9W36_RS05840 (1230718) 1230718..1231197 - 480 WP_001538397.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
G9W36_RS05845 (1231401) 1231401..1232195 - 795 WP_001538398.1 TraB/GumN family protein -
G9W36_RS05850 (1232328) 1232328..1232630 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
G9W36_RS05855 (1232666) 1232666..1233007 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
G9W36_RS05860 (1233065) 1233065..1235569 - 2505 WP_000083948.1 copper-exporting P-type ATPase CopA -
G9W36_RS05865 (1235831) 1235831..1236763 + 933 WP_001538399.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T151651 WP_000057523.1 NZ_CP049839:1232328-1232630 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T151651 NZ_CP065629:911404-911685 [Brevibacterium casei]
GTGATCAGATCGTTCGGGAGCAAGGAGACCGAACGGCTCTGGCGTCGTGAGCGCGTGCGGTCGATCGATCCGAGGATTCA
TCGGGTCGCATTGCGCAAGCTTCGTCAGGTGGGCTCAGCTGAGGTGCTCGAGGACCTCAGCGTTCCGCCGGGCAACCGGC
TCGAAGCGCTCAAGGGTGATCGCGCCGGGCAGCACAGCATCAGGATCAACGACCAGTGGCGGATCTGTTTCGTATGGACG
GACGCCGGACCAGAGGAGGTGGAGATCGTTGACTACCACTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT151651 WP_000806442.1 NZ_CP049839:1232666-1233007 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT151651 NZ_CP065629:911672-911974 [Brevibacterium casei]
TTGACTACCACTGACAAGATCGAACCGATCCACCCAGGTGAGGTCCTCATGGAGGACTTCATCGAAGGGTTCGGGATCAC
CCAGAATAAGCTCGCCGTGTCGATCGGAGTGCCGCCGCGCCGGATCAACGAGATCGTGCACGGCAAGCGTGGGATCACGG
CGGATACCGCATTGCGGCTCGCGAAGTATTTCGGGACGTCTCCGGAGTTCTGGATCAATCTGCAGAGCCACTACGAATTG
GATCGTGCGGAGGACGCCGCGGGCGAGCAAATCGCGGCGATCACCCCGCTGCGGGTTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References