Detailed information of TA system TA151649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1003260..1003954
• Replicon: chromosome
• Accession: NZ_CP049839
• Organism: Escherichia coli strain UPEC-455U/190220

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: G9W36_RS04810
• Protein ID: WP_001263500.1
• Coordinates: 1003556..1003954 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: G9W36_RS04805
• Protein ID: WP_000554758.1
• Coordinates: 1003260..1003553 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9W36_RS04780 (999077)	999077..999544	+	468	WP_000725261.1	flagellar basal body-associated FliL family protein	-
G9W36_RS04785 (999564)	999564..1000280	+	717	WP_000938731.1	FliA/WhiG family RNA polymerase sigma factor	-
G9W36_RS04790 (1000293)	1000293..1001156	+	864	WP_001169518.1	flagellar motor stator protein MotA	-
G9W36_RS04795 (1001159)	1001159..1002082	+	924	WP_001532973.1	putative lateral flagellar export/assembly protein LafU	-
G9W36_RS04800 (1002153)	1002153..1003208	+	1056	WP_001226168.1	DNA polymerase IV	-
G9W36_RS04805 (1003260)	1003260..1003553	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
G9W36_RS04810 (1003556)	1003556..1003954	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
G9W36_RS04815 (1003964)	1003964..1004416	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
G9W36_RS04820 (1004606)	1004606..1005745	+	1140	WP_000521561.1	RNA ligase RtcB family protein	-
G9W36_RS04825 (1005742)	1005742..1006356	+	615	WP_000602124.1	peptide chain release factor H	-
G9W36_RS04830 (1006413)	1006413..1007870	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
G9W36_RS04835 (1008131)	1008131..1008589	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T151649 WP_001263500.1 NZ_CP049839:1003556-1003954 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T151649 NZ_CP065627:2943824-2944225 [Delftia acidovorans]
ATGATCCTGCTCGACACCAACATCTGCATCTACATCATCAATGCCAAGCCCCCGGAGGTGCTGGCGCGCTTTCAGCAGTA
CCGCATGGGCGAGGTGGGATTGTGCAGCGTGGTGGCTGCCGAACTGGCCTTTGGCGTGGCCAAAAGCGGCTCGGCCCGTA
ACCGCCAGGCCCTGGAGATGTTTCTTGCCCCGCTCGCCATCCTCCCGTTTGACGAGGCGGCCGTGTGGGCCTATGGCGAC
CTGCGTGCCGATCTGGAGCGTCGAGGCAGTCCCATAGGCGCGCTTGACACCATGATCGCTGCCCACGCCCTCAGTCAGCG
GGCCCTGCTGGTCACCAACAACACCCGCGAATTCGCCAAGGTGCCCGGCCTGCAGTTGGACAACTGGGCTGTCGCTACCT
GA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT151649 WP_000554758.1 NZ_CP049839:1003260-1003553 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT151649 NZ_CP065627:2943594-2943827 [Delftia acidovorans]
ATGGACACCGCACGCCTTTTCCAATCCGGCCGCAGTCAAGCCGTACGGCTGCCCAAGGAATACCGTTTCACGGGCACCGA
AGTCGTGGTGAAACACTTCGGCAACGGTGTGTTGCTGCTGCCCGCAGACGACCCTTGGCAAACACTGCAAGCCGGGCTGG
CTGAATTTGAACCGGGCTTTGTGCTTGAGCGCGAGCAGCCTGAGGCCCAGCAGCGGGACGAGATCAGCCCATGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3