Detailed information of TA system TA151623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5606..6257
• Replicon: plasmid pA1254_3
• Accession: NZ_CP049809
• Organism: Acinetobacter pittii strain A1254

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: G8E09_RS19725
• Protein ID: WP_000269904.1
• Coordinates: 5606..5962 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G8E09_RS19730
• Protein ID: WP_001129974.1
• Coordinates: 5955..6257 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G8E09_RS19695	1404..2333	+	930	WP_005133531.1	replication initiation protein RepM	-
G8E09_RS19700	2412..2738	+	327	Protein_2	DNA-binding protein	-
G8E09_RS19705	2814..3654	+	841	Protein_3	replication initiation protein	-
G8E09_RS19710	3715..4266	+	552	Protein_4	DNA-binding protein	-
G8E09_RS19725	5606..5962	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G8E09_RS19730	5955..6257	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
G8E09_RS19735	6250..6459	+	210	WP_000069474.1	hypothetical protein	-
G8E09_RS19740	6634..7152	+	519	WP_000447196.1	sel1 repeat family protein	-
G8E09_RS19745	7191..7703	+	513	WP_001089572.1	SMI1/KNR4 family protein	-
G8E09_RS19750	7858..8724	-	867	WP_068562722.1	transporter	-
G8E09_RS19755	8833..10014	-	1182	WP_068562724.1	serine hydrolase	-
G8E09_RS19760	10157..11161	-	1005	WP_125276363.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11248	11248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T151623 WP_000269904.1 NZ_CP049809:5606-5962 [Acinetobacter pittii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T151623 NZ_CP065624:c3096-2941 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT151623 WP_001129974.1 NZ_CP049809:5955-6257 [Acinetobacter pittii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT151623 NZ_CP065624:3108-3166 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: N8YFZ1