Detailed information of TA system TA151609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 3833122..3833745
• Replicon: chromosome
• Accession: NZ_CP049795
• Organism: Delftia sp. WY8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: F6AKU7
• Locus tag: G4D77_RS17665
• Protein ID: WP_013800140.1
• Coordinates: 3833122..3833403 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G4D77_RS17670
• Protein ID: WP_034399070.1
• Coordinates: 3833446..3833745 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4D77_RS17640	3828356..3829219	+	864	WP_016448674.1	branched-chain amino acid ABC transporter permease	-
G4D77_RS17645	3829220..3830194	+	975	WP_046238969.1	branched-chain amino acid ABC transporter permease	-
G4D77_RS17650	3830181..3830945	+	765	WP_016452146.1	ABC transporter ATP-binding protein	-
G4D77_RS17655	3830932..3831639	+	708	WP_013800138.1	ABC transporter ATP-binding protein	-
G4D77_RS17660	3831817..3832992	+	1176	WP_016452145.1	ABC transporter substrate-binding protein	-
G4D77_RS17665	3833122..3833403	+	282	WP_013800140.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G4D77_RS17670	3833446..3833745	+	300	WP_034399070.1	HigA family addiction module antitoxin	Antitoxin
G4D77_RS17675	3833838..3834611	-	774	WP_154833118.1	SDR family oxidoreductase	-
G4D77_RS17680	3834745..3835551	-	807	WP_016448668.1	IclR family transcriptional regulator	-
G4D77_RS17685	3835596..3836369	-	774	WP_016448667.1	ferredoxin--NADP reductase	-
G4D77_RS17690	3836631..3837566	-	936	WP_154833120.1	LysR family transcriptional regulator	-
G4D77_RS17695	3837660..3838451	+	792	WP_154833122.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3427786..3837780	409994

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11067.70 Da
• Isoelectric Point: 9.3577

>T151609 WP_013800140.1 NZ_CP049795:3833122-3833403 [Delftia sp. WY8]
MTIQSFKCPDTEQLFQQRRVRRWSSIEVVALRKLRMLHAAHVLQDLRVPPGNRLEALHGDRKGQHSIRINDQWRVCFVWT
HEGPSELEIVDYH

Nucleotide

• Length: 282 bp

>T151609 NZ_CP065615:c4047084-4046809 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACAGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11023.66 Da
• Isoelectric Point: 5.0190

>AT151609 WP_034399070.1 NZ_CP049795:3833446-3833745 [Delftia sp. WY8]
MNKPLNLPTPGEILATEFLEPMGLTQYRLAQAIHVPQTRIAAIVKNGRSITADTALRLARFFGTSVEFWMNLQSQYDADM
ARKSIGDDLESIEPFALQA

Nucleotide

• Length: 300 bp

>AT151609 NZ_CP065615:c4046812-4046453 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCAATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A5P8XEH0