Detailed information of TA system TA151607

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 542689..543341
• Replicon: plasmid unnamed
• Accession: NZ_CP049794
• Organism: Ralstonia solanacearum strain 204

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0S4U5H4
• Locus tag: G7939_RS19935
• Protein ID: WP_028861315.1
• Coordinates: 542997..543341 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G7939_RS19930
• Protein ID: WP_020425351.1
• Coordinates: 542689..543000 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7939_RS19915	538261..540108	-	1848	WP_071623825.1	AAA family ATPase	-
G7939_RS19920	540521..541096	+	576	WP_071623824.1	hypothetical protein	-
G7939_RS19925	541740..542549	+	810	WP_071623829.1	DUF2242 domain-containing protein	-
G7939_RS19930	542689..543000	-	312	WP_020425351.1	transcriptional regulator	Antitoxin
G7939_RS19935	542997..543341	-	345	WP_028861315.1	hypothetical protein	Toxin
G7939_RS19940	543392..545341	-	1950	WP_023470006.1	propionate catabolism operon regulatory protein PrpR	-
G7939_RS19945	545534..546430	+	897	WP_071092368.1	methylisocitrate lyase	-
G7939_RS19950	546482..547645	+	1164	WP_016726526.1	2-methylcitrate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fliI / fliG / fliM / flgG / flgC / cyaB / motA / cheW / cheB / cheY / acrB / adeG	1..2020823	2020823

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13276.03 Da
• Isoelectric Point: 9.3395

>T151607 WP_028861315.1 NZ_CP049794:c543341-542997 [Ralstonia solanacearum]
MDATFIELPPFQRLREHYLSDDEYRALQQELLARPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIYYHWDGGGQFWM
FVVYDKGEADDLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 345 bp

>T151607 NZ_CP065615:c3000621-3000334 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11619.65 Da
• Isoelectric Point: 10.7132

>AT151607 WP_020425351.1 NZ_CP049794:c543000-542689 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKMTLRKVKVRVLQPLDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT151607 NZ_CP065615:c3000347-3000045 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A0S4U5H4