Detailed information of TA system TA151603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 324165..324817
• Replicon: plasmid unnamed
• Accession: NZ_CP049792
• Organism: Ralstonia solanacearum strain 203

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0S4U5H4
• Locus tag: G7947_RS19020
• Protein ID: WP_028861315.1
• Coordinates: 324473..324817 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G7947_RS19015
• Protein ID: WP_020425351.1
• Coordinates: 324165..324476 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7947_RS19000	319737..321584	-	1848	WP_071623825.1	AAA family ATPase	-
G7947_RS19005	321997..322572	+	576	WP_071623824.1	hypothetical protein	-
G7947_RS19010	323216..324025	+	810	WP_071623829.1	DUF2242 domain-containing protein	-
G7947_RS19015	324165..324476	-	312	WP_020425351.1	transcriptional regulator	Antitoxin
G7947_RS19020	324473..324817	-	345	WP_028861315.1	hypothetical protein	Toxin
G7947_RS19025	324868..326817	-	1950	WP_023470006.1	propionate catabolism operon regulatory protein PrpR	-
G7947_RS19030	327010..327906	+	897	WP_071092368.1	methylisocitrate lyase	-
G7947_RS19035	327958..329121	+	1164	WP_016726526.1	2-methylcitrate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	flgG / flgC / cyaB / motA / cheW / cheB / cheY / acrB / adeG / fliI / fliG / fliM	1..2013884	2013884

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13276.03 Da
• Isoelectric Point: 9.3395

>T151603 WP_028861315.1 NZ_CP049792:c324817-324473 [Ralstonia solanacearum]
MDATFIELPPFQRLREHYLSDDEYRALQQELLARPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIYYHWDGGGQFWM
FVVYDKGEADDLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 345 bp

>T151603 NZ_CP065615:2021304-2021633 [Escherichia coli]
ATGAACTACACTATCGAATACTACAGTGAAGATGTAAGGCTGGAAGTCGATCGGCTTCCATTGAGTATGCGTGCCCGATA
CCAACATCTCGTTGAACGTATGAAGATATATGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGGCTTT
TCGAACTCAGAATTAAAGGCAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTG
CATAGTTTTGTAAAGAAAACGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCA
TGACTGGTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11619.65 Da
• Isoelectric Point: 10.7132

>AT151603 WP_020425351.1 NZ_CP049792:c324476-324165 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKMTLRKVKVRVLQPLDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT151603 NZ_CP065615:2021623-2021931 [Escherichia coli]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACCCTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0S4U5H4