Detailed information of TA system TA151540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 569643..570417
• Replicon: chromosome
• Accession: NZ_CP049756
• Organism: Pasteurella multocida strain KVNON-213

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J9QLH7
• Locus tag: G7038_RS02805
• Protein ID: WP_005725755.1
• Coordinates: 569643..569969 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G7038_RS02810
• Protein ID: WP_005757699.1
• Coordinates: 570091..570417 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7038_RS02780	564959..565645	+	687	WP_104884163.1	hypothetical protein	-
G7038_RS02785	565699..567228	-	1530	WP_165544604.1	YifB family Mg chelatase-like AAA ATPase	-
G7038_RS02790	567337..567954	-	618	WP_005724253.1	ribosome biogenesis GTP-binding protein YihA/YsxC	-
G7038_RS02795	568065..568934	+	870	WP_104227942.1	VirK/YbjX family protein	-
G7038_RS02800	569009..569485	-	477	WP_104227941.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
G7038_RS02805	569643..569969	+	327	WP_005725755.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G7038_RS02810	570091..570417	+	327	WP_005757699.1	HigA family addiction module antidote protein	Antitoxin
G7038_RS02815	570472..571992	-	1521	WP_104227940.1	DUF560 domain-containing protein	-
G7038_RS02820	572051..572653	-	603	WP_005724259.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
G7038_RS02825	572704..573714	-	1011	WP_104227939.1	protein PlpE	-
G7038_RS02830	573961..574992	-	1032	WP_104227938.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (12-104)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-102)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12892.73 Da
• Isoelectric Point: 9.6705

>T151540 WP_005725755.1 NZ_CP049756:569643-569969 [Pasteurella multocida]
MDRVRFNLTEDSFQDKYLYEYFLYGSKHKNIPSDIENTLAHKLDMLDSANTLSDLRSPPGNKLEKLEPKTSGLYSIRVNV
KYRLIFKYKESNFPKITELRLDKHQYRL

Nucleotide

• Length: 327 bp

>T151540 NZ_CP065607:3110957-3111271 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12377.41 Da
• Isoelectric Point: 7.2034

>AT151540 WP_005757699.1 NZ_CP049756:570091-570417 [Pasteurella multocida]
MSRVQRKPSTVGDILLDEYLVPLNLKIADLATMLDVHRNTASALVNNNTKLSLEMALKLAKLFNTSPEFWLNLQMKLDLW
EIENNARFQESLKKISSIDQHNLLEKIA

Nucleotide

• Length: 327 bp

>AT151540 NZ_CP065607:3110721-3110954 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A2J9QLH7