Detailed information of TA system TA151535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1705358..1706004
• Replicon: chromosome
• Accession: NZ_CP049753
• Organism: Proteus mirabilis strain PmBR607

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8E4FP08
• Locus tag: G9C79_RS07920
• Protein ID: WP_060555121.1
• Coordinates: 1705657..1706004 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8E4FP64
• Locus tag: G9C79_RS07915
• Protein ID: WP_060555120.1
• Coordinates: 1705358..1705657 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9C79_RS07875	1700573..1701091	+	519	WP_060555115.1	ClbS/DfsB family four-helix bundle protein	-
G9C79_RS07880	1701560..1702183	+	624	WP_060555116.1	ParA family protein	-
G9C79_RS07885	1702180..1702386	+	207	WP_060555117.1	hypothetical protein	-
G9C79_RS07890	1702436..1702543	+	108	Protein_1518	transposase	-
G9C79_RS07895	1702537..1702611	+	75	Protein_1519	IS200/IS605 family transposase	-
G9C79_RS07900	1702798..1704288	-	1491	WP_060555118.1	YadA-like family protein	-
G9C79_RS07905	1704767..1705045	-	279	WP_060555119.1	virulence factor	-
G9C79_RS07910	1705049..1705237	-	189	WP_060555126.1	DUF5397 family protein	-
G9C79_RS07915	1705358..1705657	-	300	WP_060555120.1	XRE family transcriptional regulator	Antitoxin
G9C79_RS07920	1705657..1706004	-	348	WP_060555121.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G9C79_RS07925	1706454..1706702	+	249	WP_060555122.1	DUF883 family protein	-
G9C79_RS07930	1706774..1707127	+	354	WP_060555123.1	hypothetical protein	-
G9C79_RS07935	1707209..1707610	+	402	WP_060555124.1	phage holin family protein	-
G9C79_RS07940	1708027..1708428	-	402	WP_049218678.1	PPC domain-containing protein	-
G9C79_RS07945	1708730..1709929	+	1200	WP_049218680.1	lipoprotein-releasing ABC transporter permease subunit LolC	-
G9C79_RS07950	1710249..1710610	+	362	Protein_1530	phage holin family protein	-
G9C79_RS07955	1710607..1710900	+	294	WP_049218683.1	YqjK-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(J)	htpB	1622203..1832522	210319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13561.55 Da
• Isoelectric Point: 8.8337

>T151535 WP_060555121.1 NZ_CP049753:c1706004-1705657 [Proteus mirabilis]
MWAVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRLYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYEKLVRIAEDEFTAHLNTLESK

Nucleotide

• Length: 348 bp

>T151535 NZ_CP065607:c1931011-1930736 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11142.01 Da
• Isoelectric Point: 5.8962

>AT151535 WP_060555120.1 NZ_CP049753:c1705657-1705358 [Proteus mirabilis]
MRTLDEVIASRSPESQTRIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNELKLATLKRYIEAMG
GKLSLDVELPTGKRVAFHL

Nucleotide

• Length: 300 bp

>AT151535 NZ_CP065607:c1930739-1930380 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA