Detailed information of TA system TA151511

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4507756..4508510
• Replicon: chromosome
• Accession: NZ_CP049739
• Organism: Citrobacter sp. SX212

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: G8S20_RS21795
• Protein ID: WP_115601211.1
• Coordinates: 4507756..4508067 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G8S20_RS21800
• Protein ID: WP_253685871.1
• Coordinates: 4508064..4508510 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G8S20_RS21765 (4503423)	4503423..4504325	+	903	WP_003028691.1	formate dehydrogenase O subunit beta	-
G8S20_RS21770 (4504322)	4504322..4504957	+	636	WP_137381433.1	formate dehydrogenase cytochrome b556 subunit	-
G8S20_RS21775 (4504954)	4504954..4505883	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
G8S20_RS21780 (4505920)	4505920..4506294	-	375	WP_003028682.1	type II toxin-antitoxin system VapC family toxin	-
G8S20_RS21785 (4506294)	4506294..4506536	-	243	WP_043018956.1	CopG family transcriptional regulator	-
G8S20_RS21790 (4506741)	4506741..4507670	+	930	WP_253685870.1	alpha/beta hydrolase	-
G8S20_RS21795 (4507756)	4507756..4508067	+	312	WP_115601211.1	type II toxin-antitoxin system HigB family toxin	Toxin
G8S20_RS21800 (4508064)	4508064..4508510	+	447	WP_253685871.1	helix-turn-helix domain-containing protein	Antitoxin
G8S20_RS21805 (4508522)	4508522..4509463	-	942	WP_048214031.1	fatty acid biosynthesis protein FabY	-
G8S20_RS21810 (4509508)	4509508..4509945	-	438	WP_003028671.1	D-aminoacyl-tRNA deacylase	-
G8S20_RS21815 (4509942)	4509942..4510814	-	873	WP_121585273.1	virulence factor BrkB family protein	-
G8S20_RS21820 (4510808)	4510808..4511407	-	600	WP_048214033.1	glucose-1-phosphatase	-
G8S20_RS21825 (4511513)	4511513..4512316	-	804	WP_253685872.1	DeoR/GlpR family DNA-binding transcription regulator	-
G8S20_RS21830 (4512350)	4512350..4513246	-	897	WP_121585272.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12452.44 Da
• Isoelectric Point: 9.5344

>T151511 WP_115601211.1 NZ_CP049739:4507756-4508067 [Citrobacter sp. SX212]
VHVISRKPFNEGMLMYPNHGLALSELLNVLEKKTFHHPEEMKQYIPSLDNFKYRNKWWVIDVSGNNLRLIAYIDFRLHKV
FVKHIVLHAEYDKLTEYYRGHKE

Nucleotide

• Length: 312 bp

>T151511 NZ_CP065591:541977-542327 [Bacillus altitudinis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACGATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16699.15 Da
• Isoelectric Point: 7.4742

>AT151511 WP_253685871.1 NZ_CP049739:4508064-4508510 [Citrobacter sp. SX212]
MRSHRHMDAASAKKIVDTFSCAVKTIPLLGEERNETEYRRALALVEFLVDHDDLENPLFELLSARISEYEKHAPEFRALN
QHLKQIPSGVSVLRTLMDQYGLKATDLAKELGSKSNVSNILNGRRALTVSHIKALAERFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT151511 NZ_CP065591:541691-541972 [Bacillus altitudinis]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGGCTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA