Detailed information of TA system TA151508

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4256039..4256709
• Replicon: chromosome
• Accession: NZ_CP049739
• Organism: Citrobacter sp. SX212

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6N6JXY6
• Locus tag: G8S20_RS20645
• Protein ID: WP_046671374.1
• Coordinates: 4256407..4256709 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G8S20_RS20640
• Protein ID: WP_048213890.1
• Coordinates: 4256039..4256380 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G8S20_RS20610 (4251660)	4251660..4252418	+	759	WP_048213884.1	phosphonate C-P lyase system protein PhnK	-
G8S20_RS20615 (4252428)	4252428..4253108	+	681	WP_124021050.1	phosphonate C-P lyase system protein PhnL	-
G8S20_RS20620 (4253105)	4253105..4254241	+	1137	WP_116360962.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
G8S20_RS20625 (4254244)	4254244..4254798	+	555	WP_253685812.1	ribose 1,5-bisphosphokinase	-
G8S20_RS20630 (4254785)	4254785..4255219	+	435	WP_061067047.1	aminoalkylphosphonate N-acetyltransferase	-
G8S20_RS20635 (4255228)	4255228..4255986	+	759	WP_253685814.1	phosphonate metabolism protein PhnP	-
G8S20_RS20640 (4256039)	4256039..4256380	-	342	WP_048213890.1	HigA family addiction module antitoxin	Antitoxin
G8S20_RS20645 (4256407)	4256407..4256709	-	303	WP_046671374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G8S20_RS20650 (4256865)	4256865..4257194	-	330	WP_253685816.1	DDRRRQL repeat protein YjdP	-
G8S20_RS20655 (4257264)	4257264..4259528	-	2265	WP_124021056.1	hybrid sensor histidine kinase/response regulator	-
G8S20_RS20660 (4259643)	4259643..4261166	+	1524	WP_124021057.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11819.43 Da
• Isoelectric Point: 9.9581

>T151508 WP_046671374.1 NZ_CP049739:c4256709-4256407 [Citrobacter sp. SX212]
MSKSLNIRSFRDTWLEDFFERATSHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWVNGKAEDVYLDPHIY

Nucleotide

• Length: 303 bp

>T151508 NZ_CP065577:964401-964679 [Mycoplasma mycoides subsp. capri]
ATGATTAAGTACACAATACTTACTACAACAAAATTTATTAAAGATTTGAAGTTAGCTAAAAAACAAAATAAAAATTTAGA
AAAACTTGACATAATTATTAATAAGTTAGAAAATGATGAAATATTAGACAAAAAATATAAAGATCATCCACTCACTGGTA
AATATGAAGGTTATAGAGAATGTCATATAGAGCCTGATTGACTTTTAATTTATAAAAAGGAGAAAAAAGATCTTCTTTTA
GTACTTTTTAGGTTGGGTTCTCACTCTAAAATACTCTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13089.90 Da
• Isoelectric Point: 6.4763

>AT151508 WP_048213890.1 NZ_CP049739:c4256380-4256039 [Citrobacter sp. SX212]
MKQATRKPTTVGDILQYEYLEPLELKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQAAVDLW
EVENDMRAQEELNRIVSVKDFIAQRNSESKKVA

Nucleotide

• Length: 342 bp

>AT151508 NZ_CP065577:964136-964408 [Mycoplasma mycoides subsp. capri]
GTGAAAACAACAAATGTAAATATTAAGATGGATCCTGAAATTAAAAAACAGGCTAGTTTACTATTTAAAGAATTTGGCAT
GACCATGTCTTCTGCTATTAACCTATTTGTGAAAACTGCTGTTGAACAAAACGACATTCCATTTGAATATAATAGAGAGA
TAACTAACAAAGAAACTCTAGAAGCTTTTGAAGAAGGTGAACGTCTTTTAGCTGATAAAAACGCAAAACGTTACTCAAGT
TTTAAAGAAATATTAGACTCGTTAGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A6N6JXY6