Detailed information of TA system TA151496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4476954..4477708
• Replicon: chromosome
• Accession: NZ_CP049738
• Organism: Citrobacter sp. SX206

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: G8S19_RS21575
• Protein ID: WP_115601211.1
• Coordinates: 4476954..4477265 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G8S19_RS21580
• Protein ID: WP_253685871.1
• Coordinates: 4477262..4477708 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G8S19_RS21545 (4472621)	4472621..4473523	+	903	WP_003028691.1	formate dehydrogenase O subunit beta	-
G8S19_RS21550 (4473520)	4473520..4474155	+	636	WP_137381433.1	formate dehydrogenase cytochrome b556 subunit	-
G8S19_RS21555 (4474152)	4474152..4475081	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
G8S19_RS21560 (4475118)	4475118..4475492	-	375	WP_003028682.1	type II toxin-antitoxin system VapC family toxin	-
G8S19_RS21565 (4475492)	4475492..4475734	-	243	WP_043018956.1	CopG family transcriptional regulator	-
G8S19_RS21570 (4475939)	4475939..4476868	+	930	WP_253685870.1	alpha/beta hydrolase	-
G8S19_RS21575 (4476954)	4476954..4477265	+	312	WP_115601211.1	type II toxin-antitoxin system HigB family toxin	Toxin
G8S19_RS21580 (4477262)	4477262..4477708	+	447	WP_253685871.1	helix-turn-helix domain-containing protein	Antitoxin
G8S19_RS21585 (4477720)	4477720..4478661	-	942	WP_048214031.1	fatty acid biosynthesis protein FabY	-
G8S19_RS21590 (4478706)	4478706..4479143	-	438	WP_003028671.1	D-aminoacyl-tRNA deacylase	-
G8S19_RS21595 (4479140)	4479140..4480012	-	873	WP_121585273.1	virulence factor BrkB family protein	-
G8S19_RS21600 (4480006)	4480006..4480605	-	600	WP_048214033.1	glucose-1-phosphatase	-
G8S19_RS21605 (4480711)	4480711..4481514	-	804	WP_253685872.1	DeoR/GlpR family DNA-binding transcription regulator	-
G8S19_RS21610 (4481548)	4481548..4482444	-	897	WP_121585272.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12452.44 Da
• Isoelectric Point: 9.5344

>T151496 WP_115601211.1 NZ_CP049738:4476954-4477265 [Citrobacter sp. SX206]
VHVISRKPFNEGMLMYPNHGLALSELLNVLEKKTFHHPEEMKQYIPSLDNFKYRNKWWVIDVSGNNLRLIAYIDFRLHKV
FVKHIVLHAEYDKLTEYYRGHKE

Nucleotide

• Length: 312 bp

>T151496 NZ_CP065554:4203489-4203707 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16699.15 Da
• Isoelectric Point: 7.4742

>AT151496 WP_253685871.1 NZ_CP049738:4477262-4477708 [Citrobacter sp. SX206]
MRSHRHMDAASAKKIVDTFSCAVKTIPLLGEERNETEYRRALALVEFLVDHDDLENPLFELLSARISEYEKHAPEFRALN
QHLKQIPSGVSVLRTLMDQYGLKATDLAKELGSKSNVSNILNGRRALTVSHIKALAERFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT151496 NZ_CP065554:4203088-4203462 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG