Detailed information of TA system TA151493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4225237..4225907
• Replicon: chromosome
• Accession: NZ_CP049738
• Organism: Citrobacter sp. SX206

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6N6JXY6
• Locus tag: G8S19_RS20430
• Protein ID: WP_046671374.1
• Coordinates: 4225605..4225907 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G8S19_RS20425
• Protein ID: WP_048213890.1
• Coordinates: 4225237..4225578 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G8S19_RS20395 (4220858)	4220858..4221616	+	759	WP_048213884.1	phosphonate C-P lyase system protein PhnK	-
G8S19_RS20400 (4221626)	4221626..4222306	+	681	WP_124021050.1	phosphonate C-P lyase system protein PhnL	-
G8S19_RS20405 (4222303)	4222303..4223439	+	1137	WP_116360962.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
G8S19_RS20410 (4223442)	4223442..4223996	+	555	WP_253685812.1	ribose 1,5-bisphosphokinase	-
G8S19_RS20415 (4223983)	4223983..4224417	+	435	WP_061067047.1	aminoalkylphosphonate N-acetyltransferase	-
G8S19_RS20420 (4224426)	4224426..4225184	+	759	WP_253685814.1	phosphonate metabolism protein PhnP	-
G8S19_RS20425 (4225237)	4225237..4225578	-	342	WP_048213890.1	HigA family addiction module antitoxin	Antitoxin
G8S19_RS20430 (4225605)	4225605..4225907	-	303	WP_046671374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G8S19_RS20435 (4226063)	4226063..4226392	-	330	WP_253685816.1	DDRRRQL repeat protein YjdP	-
G8S19_RS20440 (4226462)	4226462..4228726	-	2265	WP_124021056.1	hybrid sensor histidine kinase/response regulator	-
G8S19_RS20445 (4228841)	4228841..4230364	+	1524	WP_124021057.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11819.43 Da
• Isoelectric Point: 9.9581

>T151493 WP_046671374.1 NZ_CP049738:c4225907-4225605 [Citrobacter sp. SX206]
MSKSLNIRSFRDTWLEDFFERATSHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWVNGKAEDVYLDPHIY

Nucleotide

• Length: 303 bp

>T151493 NZ_CP065554:876831-877238 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13089.90 Da
• Isoelectric Point: 6.4763

>AT151493 WP_048213890.1 NZ_CP049738:c4225578-4225237 [Citrobacter sp. SX206]
MKQATRKPTTVGDILQYEYLEPLELKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQAAVDLW
EVENDMRAQEELNRIVSVKDFIAQRNSESKKVA

Nucleotide

• Length: 342 bp

>AT151493 NZ_CP065554:876584-876850 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A6N6JXY6