Detailed information of TA system TA151417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 333349..333940
• Replicon: chromosome
• Accession: NZ_CP049684
• Organism: Polynucleobacter paneuropaeus strain AP-Creno-5A-D2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C2742_RS01805
• Protein ID: WP_112203122.1
• Coordinates: 333349..333627 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C2742_RS01810
• Protein ID: WP_215272412.1
• Coordinates: 333635..333940 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2742_RS01780 (C2742_01780)	328692..329711	-	1020	WP_215278909.1	ionic transporter y4hA	-
C2742_RS01785 (C2742_01785)	329782..330144	+	363	WP_215278910.1	CbiX/SirB N-terminal domain-containing protein	-
C2742_RS01790 (C2742_01790)	330067..330879	-	813	WP_215279087.1	uroporphyrinogen-III C-methyltransferase	-
C2742_RS01795 (C2742_01795)	330891..331481	-	591	WP_215278911.1	DUF934 domain-containing protein	-
C2742_RS01800 (C2742_01800)	331478..333178	-	1701	WP_215278912.1	nitrite/sulfite reductase	-
C2742_RS01805 (C2742_01805)	333349..333627	+	279	WP_112203122.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2742_RS01810 (C2742_01810)	333635..333940	+	306	WP_215272412.1	HigA family addiction module antitoxin	Antitoxin
C2742_RS01815 (C2742_01815)	333991..334239	+	249	WP_112203124.1	prevent-host-death family protein	-
C2742_RS01820 (C2742_01820)	334323..334682	-	360	WP_215278913.1	hypothetical protein	-
C2742_RS01825 (C2742_01825)	334696..334917	-	222	WP_112208850.1	hypothetical protein	-
C2742_RS01830 (C2742_01830)	335089..335682	+	594	WP_215278914.1	DUF308 domain-containing protein	-
C2742_RS01835 (C2742_01835)	335725..335895	+	171	WP_158525527.1	hypothetical protein	-
C2742_RS01840 (C2742_01840)	335911..338067	+	2157	WP_215278915.1	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-91)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10686.35 Da
• Isoelectric Point: 10.1614

>T151417 WP_112203122.1 NZ_CP049684:333349-333627 [Polynucleobacter paneuropaeus]
MIHSFLCGLTKDLFDSKPSKKFGNIERAARRKLLQLHAATAISDLRTPPGNMLELLIGNRRGQYSIRINHQWRICFVWRE
DGAHNVEIVDYH

Nucleotide

• Length: 279 bp

>T151417 NZ_CP065464:c1115850-1115632 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11701.62 Da
• Isoelectric Point: 5.7251

>AT151417 WP_215272412.1 NZ_CP049684:333635-333940 [Polynucleobacter paneuropaeus]
MTTKLLDEIHPGEILLEDFMKPMGITSRQLAADIDVSPSRISEIIHGTRPITADTALRLGLFFSMEPRFWLNLQSEYDMR
MAKRNLQEEIEPRIRVFRIAA

Nucleotide

• Length: 306 bp

>AT151417 NZ_CP065464:c1116251-1115877 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG