Detailed information of TA system TA151413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 320242..320833
• Replicon: chromosome
• Accession: NZ_CP049683
• Organism: Polynucleobacter paneuropaeus strain AP-RePozz10-5-G5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C2754_RS01740
• Protein ID: WP_112203122.1
• Coordinates: 320242..320520 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C2754_RS01745
• Protein ID: WP_215272412.1
• Coordinates: 320528..320833 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2754_RS01715 (C2754_01715)	315469..316497	-	1029	WP_215286139.1	ionic transporter y4hA	-
C2754_RS01720 (C2754_01720)	316597..316959	+	363	WP_215286140.1	CbiX/SirB N-terminal domain-containing protein	-
C2754_RS01725 (C2754_01725)	316882..317730	-	849	WP_215272148.1	uroporphyrinogen-III C-methyltransferase	-
C2754_RS01730 (C2754_01730)	317759..318349	-	591	WP_215272149.1	DUF934 domain-containing protein	-
C2754_RS01735 (C2754_01735)	318346..320070	-	1725	WP_215286141.1	nitrite/sulfite reductase	-
C2754_RS01740 (C2754_01740)	320242..320520	+	279	WP_112203122.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2754_RS01745 (C2754_01745)	320528..320833	+	306	WP_215272412.1	HigA family addiction module antitoxin	Antitoxin
C2754_RS01750 (C2754_01750)	320884..321132	+	249	WP_112203124.1	prevent-host-death family protein	-
C2754_RS01755 (C2754_01755)	321216..321575	-	360	WP_215278913.1	hypothetical protein	-
C2754_RS01760 (C2754_01760)	321589..321810	-	222	WP_112208850.1	hypothetical protein	-
C2754_RS01765 (C2754_01765)	321982..322575	+	594	WP_215278914.1	DUF308 domain-containing protein	-
C2754_RS01770 (C2754_01770)	322618..322788	+	171	WP_158525527.1	hypothetical protein	-
C2754_RS01775 (C2754_01775)	322804..324960	+	2157	WP_215278915.1	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-91)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10686.35 Da
• Isoelectric Point: 10.1614

>T151413 WP_112203122.1 NZ_CP049683:320242-320520 [Polynucleobacter paneuropaeus]
MIHSFLCGLTKDLFDSKPSKKFGNIERAARRKLLQLHAATAISDLRTPPGNMLELLIGNRRGQYSIRINHQWRICFVWRE
DGAHNVEIVDYH

Nucleotide

• Length: 279 bp

>T151413 NZ_CP065463:24691-25041 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11701.62 Da
• Isoelectric Point: 5.7251

>AT151413 WP_215272412.1 NZ_CP049683:320528-320833 [Polynucleobacter paneuropaeus]
MTTKLLDEIHPGEILLEDFMKPMGITSRQLAADIDVSPSRISEIIHGTRPITADTALRLGLFFSMEPRFWLNLQSEYDMR
MAKRNLQEEIEPRIRVFRIAA

Nucleotide

• Length: 306 bp

>AT151413 NZ_CP065463:25046-25348 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA