Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3750220..3750947 Replicon chromosome
Accession NZ_CP049606
Organism Shigella boydii strain 600080

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag G5S61_RS18930 Protein ID WP_000550189.1
Coordinates 3750633..3750947 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag G5S61_RS18925 Protein ID WP_000560266.1
Coordinates 3750220..3750636 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5S61_RS18915 (3745580) 3745580..3747931 + 2352 WP_039066588.1 alpha-glucosidase -
G5S61_RS18920 (3748157) 3748157..3750175 + 2019 WP_039066590.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
G5S61_RS18925 (3750220) 3750220..3750636 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
G5S61_RS18930 (3750633) 3750633..3750947 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
G5S61_RS18935 (3751232) 3751232..3752368 - 1137 WP_039066593.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
G5S61_RS18940 (3752453) 3752453..3752956 + 504 WP_001295542.1 M48 family metallopeptidase -
G5S61_RS18945 (3753033) 3753033..3753725 + 693 WP_000942537.1 vancomycin high temperature exclusion protein -
G5S61_RS18950 (3753804) 3753804..3754802 + 999 WP_005050984.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T151381 WP_000550189.1 NZ_CP049606:c3750947-3750633 [Shigella boydii]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T151381 NZ_CP065453:c4019972-4019754 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT151381 WP_000560266.1 NZ_CP049606:c3750636-3750220 [Shigella boydii]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT151381 NZ_CP065453:c4020373-4019999 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References