Detailed information of TA system TA151379

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3396240..3396967
• Replicon: chromosome
• Accession: NZ_CP049606
• Organism: Shigella boydii strain 600080

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4P7TJ48
• Locus tag: G5S61_RS17210
• Protein ID: WP_000547561.1
• Coordinates: 3396656..3396967 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G5S61_RS17205
• Protein ID: WP_000126296.1
• Coordinates: 3396240..3396659 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5S61_RS17185 (3391289)	3391289..3391816	-	528	WP_001078777.1	electron transport protein HydN	-
G5S61_RS17190 (3391965)	3391965..3392975	-	1011	WP_005051459.1	DNA-binding transcriptional regulator AscG	-
G5S61_RS17195 (3393235)	3393235..3394692	+	1458	WP_039066436.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
G5S61_RS17200 (3394701)	3394701..3396125	+	1425	WP_039066464.1	6-phospho-beta-glucosidase AscB	-
G5S61_RS17205 (3396240)	3396240..3396659	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
G5S61_RS17210 (3396656)	3396656..3396967	-	312	WP_000547561.1	type II toxin-antitoxin system HigB family toxin	Toxin
G5S61_RS17215 (3397140)	3397140..3397883	-	744	WP_039066435.1	hypothetical protein	-
G5S61_RS17220 (3397926)	3397926..3398396	-	471	WP_039066434.1	hydrogenase maturation peptidase HycI	-
G5S61_RS17225 (3398389)	3398389..3398799	-	411	WP_001291916.1	formate hydrogenlyase assembly protein HycH	-
G5S61_RS17230 (3398796)	3398796..3399563	-	768	WP_000067392.1	formate hydrogenlyase subunit HycG	-
G5S61_RS17235 (3399563)	3399563..3400105	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
G5S61_RS17240 (3400115)	3400115..3401756	-	1642	Protein_3407	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12370.06 Da
• Isoelectric Point: 9.7105

>T151379 WP_000547561.1 NZ_CP049606:c3396967-3396656 [Shigella boydii]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDAGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T151379 NZ_CP065453:3305352-3305636 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT151379 WP_000126296.1 NZ_CP049606:c3396659-3396240 [Shigella boydii]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT151379 NZ_CP065453:3305120-3305362 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A4P7TJ48