Detailed information of TA system TA151376

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2620552..2620777
• Replicon: chromosome
• Accession: NZ_CP049606
• Organism: Shigella boydii strain 600080

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: G5S61_RS13430
• Protein ID: WP_000813254.1
• Coordinates: 2620552..2620707 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2620719..2620777 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5S61_RS13370	2615864..2616214	-	351	WP_005049241.1	IS66 family insertion sequence element accessory protein TnpB	-
G5S61_RS13375	2616211..2616885	-	675	WP_039065696.1	IS66-like element accessory protein TnpA	-
G5S61_RS13380	2616984..2617199	-	216	WP_000839572.1	class II holin family protein	-
G5S61_RS13405	2617995..2618683	-	689	Protein_2653	bacteriophage antitermination protein Q	-
G5S61_RS13410	2618680..2619045	-	366	WP_000140020.1	RusA family crossover junction endodeoxyribonuclease	-
G5S61_RS13415	2619046..2620104	-	1059	WP_039066246.1	DUF968 domain-containing protein	-
G5S61_RS13420	2620106..2620384	-	279	WP_011069426.1	hypothetical protein	-
G5S61_RS13430	2620552..2620707	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	2620719..2620777	+	59	-	-	Antitoxin
G5S61_RS13435	2621359..2621643	-	285	Protein_2658	hypothetical protein	-
G5S61_RS23720	2622255..2622443	+	189	WP_260479634.1	hypothetical protein	-
G5S61_RS13445	2622389..2622565	+	177	WP_039066248.1	DUF4224 domain-containing protein	-
G5S61_RS13450	2622565..2623605	+	1041	WP_005096324.1	tyrosine-type recombinase/integrase	-
G5S61_RS13460	2623816..2624613	+	798	WP_039066256.1	DgsA anti-repressor MtfA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH9.8	2595741..2629521	33780
	-	inside	IScluster/Tn	-	-	2613844..2629271	15427

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T151376 WP_000813254.1 NZ_CP049606:c2620707-2620552 [Shigella boydii]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T151376 NZ_CP065453:c2422906-2422538 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT151376 NZ_CP049606:2620719-2620777 [Shigella boydii]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin