Detailed information of TA system TA151365

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1962777..1963002
• Replicon: chromosome
• Accession: NZ_CP049606
• Organism: Shigella boydii strain 600080

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: G5S61_RS09845
• Protein ID: WP_000813254.1
• Coordinates: 1962847..1963002 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1962777..1962835 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5S61_RS09800	1957803..1958251	-	449	Protein_1942	transposase	-
G5S61_RS23610	1958775..1958944	+	170	Protein_1943	hypothetical protein	-
G5S61_RS09810	1959090..1959836	+	747	WP_000788996.1	ATP-binding protein	-
G5S61_RS09815	1959851..1960273	+	423	WP_001118167.1	DUF977 family protein	-
G5S61_RS09820	1960331..1960687	+	357	WP_005048249.1	hypothetical protein	-
G5S61_RS09825	1960780..1960998	+	219	WP_000256998.1	DUF4014 family protein	-
G5S61_RS09830	1961000..1961365	+	366	WP_001229298.1	HNH endonuclease signature motif containing protein	-
G5S61_RS09835	1961362..1962027	+	666	WP_000208062.1	hypothetical protein	-
G5S61_RS09840	1962027..1962392	+	366	WP_000610655.1	DUF551 domain-containing protein	-
-	1962777..1962835	-	59	-	-	Antitoxin
G5S61_RS09845	1962847..1963002	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
G5S61_RS09855	1964104..1964436	+	333	WP_171764399.1	hypothetical protein	-
G5S61_RS09860	1964339..1964938	+	600	WP_000940329.1	DUF1367 family protein	-
G5S61_RS09865	1964938..1965228	+	291	WP_000228038.1	DUF1364 domain-containing protein	-
G5S61_RS09870	1965225..1965779	+	555	WP_000640143.1	DUF1133 family protein	-
G5S61_RS09875	1965932..1966105	+	174	WP_000504450.1	hypothetical protein	-
G5S61_RS09880	1966167..1967323	+	1157	WP_128600754.1	IS3-like element IS600 family transposase	-
G5S61_RS09885	1967316..1967876	+	561	WP_072075205.1	ORF6N domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	1953594..2013582	59988

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T151365 WP_000813254.1 NZ_CP049606:1962847-1963002 [Shigella boydii]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T151365 NZ_CP065447:c3998359-3997976 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT151365 NZ_CP049606:c1962835-1962777 [Shigella boydii]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin