Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 816648..817342 Replicon chromosome
Accession NZ_CP049606
Organism Shigella boydii strain 600080

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag G5S61_RS03900 Protein ID WP_001263491.1
Coordinates 816648..817046 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag G5S61_RS03905 Protein ID WP_000554759.1
Coordinates 817049..817342 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5S61_RS03875 (811987) 811987..812445 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
G5S61_RS03880 (812707) 812707..814164 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
G5S61_RS03885 (814221) 814221..814781 - 561 Protein_774 peptide chain release factor H -
G5S61_RS03890 (814897) 814897..815940 - 1044 WP_005060486.1 RNA ligase RtcB family protein -
G5S61_RS03895 (816186) 816186..816638 - 453 WP_001059862.1 GNAT family N-acetyltransferase -
G5S61_RS03900 (816648) 816648..817046 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
G5S61_RS03905 (817049) 817049..817342 - 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
G5S61_RS03910 (817394) 817394..818449 - 1056 WP_001226172.1 DNA polymerase IV -
G5S61_RS03915 (818520) 818520..819305 - 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
G5S61_RS03920 (819277) 819277..820989 + 1713 Protein_781 flagellar biosynthesis protein FlhA -
G5S61_RS03925 (821112) 821112..821609 - 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
G5S61_RS03930 (821785) 821785..822306 - 522 Protein_783 C40 family peptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T151358 WP_001263491.1 NZ_CP049606:c817046-816648 [Shigella boydii]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T151358 NZ_CP065446:136704-137054 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT151358 WP_000554759.1 NZ_CP049606:c817342-817049 [Shigella boydii]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT151358 NZ_CP065446:137059-137361 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References