Detailed information of TA system TA151311

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 134223..134456
• Replicon: plasmid pT28R-2
• Accession: NZ_CP049355
• Organism: Escherichia coli strain T28R

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: G7B60_RS23985
• Protein ID: WP_001312861.1
• Coordinates: 134223..134381 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	134425..134456 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7B60_RS23955	129597..130286	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
G7B60_RS23960	130473..130856	-	384	WP_001151524.1	relaxosome protein TraM	-
G7B60_RS23965	131177..131779	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
G7B60_RS23970	132076..132897	-	822	WP_001234426.1	DUF945 domain-containing protein	-
G7B60_RS23975	133015..133302	-	288	WP_000107535.1	hypothetical protein	-
G7B60_RS23985	134223..134381	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	134425..134456	-	32	NuclAT_1	-	Antitoxin
-	134425..134456	-	32	NuclAT_1	-	Antitoxin
-	134425..134456	-	32	NuclAT_1	-	Antitoxin
-	134425..134456	-	32	NuclAT_1	-	Antitoxin
-	135898..136095	-	198	NuclAT_0	-	-
-	135898..136095	-	198	NuclAT_0	-	-
-	135898..136095	-	198	NuclAT_0	-	-
-	135898..136095	-	198	NuclAT_0	-	-
G7B60_RS23995	135907..136095	+	189	WP_001299721.1	hypothetical protein	-
G7B60_RS24000	136107..136826	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
G7B60_RS24005	136823..137257	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
G7B60_RS24010	137312..137509	-	198	Protein_151	hypothetical protein	-
G7B60_RS24015	137537..137770	-	234	WP_000005987.1	DUF905 family protein	-
G7B60_RS24020	137838..138377	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
G7B60_RS24615	138403..138609	-	207	WP_000275856.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14 / fosA3 / aac(3)-IVa / sul2 / floR / aph(3')-Ia / mph(A) / qacE / aadA2 / dfrA12 / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..161057	161057

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T151311 WP_001312861.1 NZ_CP049355:c134381-134223 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T151311 NZ_CP065436:c3446569-3446285 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Nucleotide

• Length: 32 bp

>AT151311 NZ_CP049355:c134456-134425 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin