Detailed information of TA system TA151301

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3639921..3640615
• Replicon: chromosome
• Accession: NZ_CP049353
• Organism: Escherichia coli strain T28R

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: G7B60_RS17615
• Protein ID: WP_001263493.1
• Coordinates: 3639921..3640319 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: G7B60_RS17620
• Protein ID: WP_152927302.1
• Coordinates: 3640322..3640615 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3634979..3635059	-	81	NuclAT_8	-	-
-	3634979..3635059	-	81	NuclAT_8	-	-
-	3634979..3635059	-	81	NuclAT_8	-	-
-	3634979..3635059	-	81	NuclAT_8	-	-
G7B60_RS17585	3635655..3636113	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
G7B60_RS17590	3636374..3637831	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
G7B60_RS17595	3637888..3638409	-	522	Protein_3434	peptide chain release factor H	-
G7B60_RS17600	3638408..3638611	-	204	Protein_3435	RNA ligase RtcB family protein	-
G7B60_RS17605	3638869..3639566	-	698	Protein_3436	IS1 family transposase	-
G7B60_RS17610	3639621..3639911	-	291	Protein_3437	GNAT family N-acetyltransferase	-
G7B60_RS17615	3639921..3640319	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
G7B60_RS17620	3640322..3640615	-	294	WP_152927302.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
G7B60_RS17625	3640667..3641722	-	1056	WP_001226164.1	DNA polymerase IV	-
G7B60_RS17630	3641793..3642578	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
G7B60_RS17635	3642550..3644262	+	1713	Protein_3442	flagellar biosynthesis protein FlhA	-
G7B60_RS17640	3644486..3644983	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	3606573..3670098	63525
	-	flank	IS/Tn	-	-	3638869..3639372	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T151301 WP_001263493.1 NZ_CP049353:c3640319-3639921 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T151301 NZ_CP065431:1950248-1951093 [Streptococcus suis]
ATGAATAAAGCTAAGCTAACAGCACTCTGTCATAAAATATCAAAGAATACTGGATTAACCTTTAATTCCGTGATGACCTA
TTACTTTTTAGAAATGATTTTGAAAAAATTAAGTCAGAGTGCCTACTCAAATCACTATATCTTCAAAGGAGGATTCCTAC
TGTCAAATGTTATTGGAGTTGAATCTCGTAGCACGGTTGATATTGATTTTTTGTTCCATCAAATGACACTCTCAGAAGAT
ACTGTGAAGCAGCAACTCAAGGAAATTTTAGTAGATTCAGAAGAAGGTATATCTTTTGTGATTCAATCAATCAAAGCTAT
TAAAGAAAGTGATGACTATGGTGGCTATCGAGCAACAATTTTGTGCCAACTTGAGAATATTAAACAAGTTATTCATTTGG
ATATTGCGACAGGCGATGTTGTAACTCCTCAGCCGATTACATACAACTATAAAGCTATTTTTGATGAAGACAATTTTCCA
ATCATTGCTTATACGATTGAAACAATTCTAGCTGAGAAACTTCAAACCATCTATTCACGCAACTTCTTGAACAGCAGAAG
CAAAGATTTTTACGATGTTTATATTCTTTCAAAACTGAAGAAAGAAGACATTGACTTCATTCAGTTGAGAAATGCCTGTC
AGAGAACATTTTCATATCGCGAAACAGAACTCGATTTTGTAAAGATTATTGAACTACTCGAGAGGTTCAAATCTGACCCT
ATTCAGAATAAACAATGGCAAAATTATTCAAAAAAATATAGCTATACAAAAGGGATTTCACTTGCAGATGTTCTTGATGA
GATGATTAGTCTTATTACAATTATTATTTCTACAGACTTAGATTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11180.76 Da
• Isoelectric Point: 5.2168

>AT151301 WP_152927302.1 NZ_CP049353:c3640615-3640322 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKACFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT151301 NZ_CP065431:1949661-1950251 [Streptococcus suis]
ATGTCAAAAAAAGAGATTCTACTTGAATTTATAGAAAACCACAATGGTATTATCACCTATAGAGATTGTAAAGCTCTAAA
TATTCCAACAATATATTTGACAAGACTAGAAAAAGAAGGAATTCTATACCGCGTCGAAAAAGGAATTTTCCTAACCCAGA
ATGGAGACTACGACGAATACTACTTCTTTCAGTACCGATATCCCAAGGCTGTTTTCTCATATATCTCAGCGCTTTACCTG
CAACAATTTACAGATGAAATTCCTCAATATTTTGACGTAACCATTCCTAGAGGTTACCGTTTTAATACTCCTCCTGCTAA
CCTGAACATTCATTCCGTTTCTAAGGAATATAGCGAACTAGGAATTACTCTTGTAAAGACCCCTATGGGAAACGAAGTAA
GAGTGCATGATTTCGAACGTATTATCTGTGATTTTGTGATTCATCGAGAAAAAATAGATACTGAGCTTTTTGTCAAAACA
CTTCAACATTATGGAAACTATCCTAAAAAAAATCTTACAAAACTCTATGAATATGCGACAAAAATGAACACCTTGGACAA
GGTCAAACAAACTCTGGAGGTCCTAGTATGA

Structures

Toxin

• AlphaFold DB: S1EXB8