151283

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 644515..645242 Replicon chromosome
Accession NZ_CP049353
Organism Escherichia coli strain T28R

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag G7B60_RS03065 Protein ID WP_000550189.1
Coordinates 644515..644829 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag G7B60_RS03070 Protein ID WP_000560266.1
Coordinates 644826..645242 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G7B60_RS03045 640673..641659 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
G7B60_RS03050 641738..642430 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
G7B60_RS03055 642507..643010 - 504 WP_001300832.1 M48 family metallopeptidase -
G7B60_RS03060 643095..644231 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
G7B60_RS03065 644515..644829 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
G7B60_RS03070 644826..645242 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
G7B60_RS03075 645287..647305 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
G7B60_RS03080 647731..650082 - 2352 WP_000695486.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T151283 WP_000550189.1 NZ_CP049353:644515-644829 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T151283 NZ_CP065420:1558230-1558640 [Providencia sp. 2.29]
GTGGTCCTGTGGAAATCTAATCTCTCCATTTCATGGAAAACTCAGCTTTTTTCAACCTGTGCTCATGGGGTAGTCGGCTT
TATTCTATTGGTCGCACCATGGGCGCCAGGGAATTCAATGGTGTGGTTGCCGCTGTTAGTGATTGTGATTGCTAGCTGGG
CAAAAAGCCAAAAAAGCATCAGCAAAATCAAAGGGACTGCGGTACTGGTTAATGGCAATAAAGTCCAATGGAAAAAGAAT
GAGTGGAGTATCGTTAAGCAACCTTGGTGTAGCCGTGTGGGGGTATTATTGACACTCAGTGCCTTACAAGGTAAGCCACA
GAAGATCCGTTTATGGGTTGCAAAAGATGCCTTATCAGAAGAGAGCTGGCGTAATTTAAACCAACTATTACTGCAATATC
CTGATATTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT151283 WP_000560266.1 NZ_CP049353:644826-645242 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT151283 NZ_CP065420:1557983-1558249 [Providencia sp. 2.29]
ATGGATATAGAGAATAAAGCAAGAATTCATTGGGCTTGCCGTCGTGGTATGCGTGAGTTGGATATTTCCATCATGCCATT
CTTTGAATATGAATATGAAGGACTTTCTGACAAGGACAAACGTACTTTTGTTCGTTTACTTGAGTGTGCAGACCCTGATC
TGTTTAACTGGTTAATGAATCATGGGCGCCCAGACGACGATGAGTTGTTTGGTATGGTTAAATTGATTCAGGAAAGAAAT
AAAAATCGTGGTCCTGTGGAAATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References