Detailed information of TA system TA151279

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39585..39838
• Replicon: plasmid p3R-3
• Accession: NZ_CP049351
• Organism: Escherichia coli strain 3R

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: G6Q95_RS24950
• Protein ID: WP_001312851.1
• Coordinates: 39585..39734 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	39779..39838 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Q95_RS24920	35942..36199	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
G6Q95_RS24925	36292..36945	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
G6Q95_RS24930	37043..37183	-	141	WP_001333237.1	hypothetical protein	-
G6Q95_RS24935	37884..38741	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
G6Q95_RS24940	38734..38808	-	75	WP_001375168.1	RepA leader peptide Tap	-
G6Q95_RS24945	39053..39301	-	249	WP_000083837.1	replication regulatory protein RepA	-
G6Q95_RS24950	39585..39734	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	39779..39838	+	60	NuclAT_1	-	Antitoxin
-	39779..39838	+	60	NuclAT_1	-	Antitoxin
-	39779..39838	+	60	NuclAT_1	-	Antitoxin
-	39779..39838	+	60	NuclAT_1	-	Antitoxin
G6Q95_RS24955	40433..41032	-	600	WP_032083981.1	hypothetical protein	-
G6Q95_RS24960	41418..41618	-	201	WP_015059022.1	hypothetical protein	-
G6Q95_RS24965	41750..42310	-	561	WP_000139328.1	fertility inhibition protein FinO	-
G6Q95_RS24970	42365..42847	-	483	Protein_60	type-F conjugative transfer system pilin acetylase TraX	-
G6Q95_RS24975	42853..43395	-	543	Protein_61	conjugative relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	rmtB / blaTEM-1B	-	1..68949	68949

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T151279 WP_001312851.1 NZ_CP049351:c39734-39585 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T151279 NZ_CP065417:c5653174-5652875 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT151279 NZ_CP049351:39779-39838 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin