Detailed information of TA system TA151275

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 9053..9479
• Replicon: plasmid p3R-3
• Accession: NZ_CP049351
• Organism: Escherichia coli strain 3R

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: G6Q95_RS24735
• Protein ID: WP_001312861.1
• Coordinates: 9053..9211 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	9255..9479 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Q95_RS24695	4108..4335	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
G6Q95_RS24700	4429..5115	-	687	WP_001825184.1	PAS domain-containing protein	-
G6Q95_RS24705	5306..5689	-	384	WP_000124981.1	relaxosome protein TraM	-
G6Q95_RS24710	5966..6613	+	648	WP_001825185.1	transglycosylase SLT domain-containing protein	-
G6Q95_RS24715	6910..7731	-	822	WP_001234437.1	DUF945 domain-containing protein	-
G6Q95_RS24720	7842..8138	-	297	WP_001272251.1	hypothetical protein	-
G6Q95_RS25550	8438..8815	+	378	Protein_14	hypothetical protein	-
G6Q95_RS24735	9053..9211	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	9255..9479	-	225	NuclAT_0	-	Antitoxin
-	9255..9479	-	225	NuclAT_0	-	Antitoxin
-	9255..9479	-	225	NuclAT_0	-	Antitoxin
-	9255..9479	-	225	NuclAT_0	-	Antitoxin
G6Q95_RS24740	9291..9470	+	180	WP_001309233.1	hypothetical protein	-
G6Q95_RS24745	9491..10210	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
G6Q95_RS24750	10207..10641	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
G6Q95_RS24755	10710..12733	-	2024	Protein_19	ParB/RepB/Spo0J family partition protein	-
G6Q95_RS24760	12794..13027	-	234	WP_000006003.1	DUF905 family protein	-
G6Q95_RS24765	13085..13612	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
G6Q95_RS25555	13914..14369	+	456	WP_001424978.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	rmtB / blaTEM-1B	-	1..68949	68949

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T151275 WP_001312861.1 NZ_CP049351:c9211-9053 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T151275 NZ_CP065417:3717364-3717942 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCCGCGCACCCGGACAAAGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCACCTCGACGC
GCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGTGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT151275 NZ_CP049351:c9479-9255 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin