Detailed information of TA system TA151269

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 14713..14977
• Replicon: plasmid p3R-2
• Accession: NZ_CP049350
• Organism: Escherichia coli strain 3R

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: G6Q95_RS24175
• Protein ID: WP_001303307.1
• Coordinates: 14713..14865 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	14915..14977 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Q95_RS24145	9996..10658	+	663	WP_000644794.1	plasmid IncI1-type surface exclusion protein ExcA	-
G6Q95_RS24150	10730..10939	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
G6Q95_RS24155	11331..11507	+	177	WP_001054900.1	hypothetical protein	-
-	11993..12044	+	52	NuclAT_1	-	-
-	11993..12044	+	52	NuclAT_1	-	-
-	11993..12044	+	52	NuclAT_1	-	-
-	11993..12044	+	52	NuclAT_1	-	-
G6Q95_RS24160	12228..13250	+	1023	WP_001572805.1	IS21-like element IS100 family transposase	-
G6Q95_RS24165	13247..14029	+	783	WP_001300609.1	IS21-like element IS100kyp family helper ATPase IstB	-
G6Q95_RS24170	14390..14641	+	252	WP_001291965.1	hypothetical protein	-
G6Q95_RS24175	14713..14865	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
-	14915..14977	+	63	NuclAT_0	-	Antitoxin
-	14915..14977	+	63	NuclAT_0	-	Antitoxin
-	14915..14977	+	63	NuclAT_0	-	Antitoxin
-	14915..14977	+	63	NuclAT_0	-	Antitoxin
G6Q95_RS24180	15157..16365	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
G6Q95_RS24185	16384..17454	+	1071	WP_000151575.1	IncI1-type conjugal transfer protein TrbB	-
G6Q95_RS24190	17447..19738	+	2292	WP_001289271.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-2	-	1..94989	94989

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T151269 WP_001303307.1 NZ_CP049350:c14865-14713 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T151269 NZ_CP065411:4808248-4808430 [Kosakonia radicincitans]
ATGAAATACAGCGAGTTCAGAAAGTGGTTGTTATCTCAGGGAGCGGAGCTAACCAAAGCTCCAGGTGGAGGAAGCCATTT
TAAAGTCAGGCTTAATGGTAGGGCATCGGTTTTCCCTTTTCATGGTTCGAAAGAGATTCCTGAGCCGTTGAGGAAAAAGA
TTATGAAGGACTTAGGCCTGTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT151269 NZ_CP049350:14915-14977 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin