Detailed information of TA system TA151266

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 170483..170737
• Replicon: plasmid p3R-1
• Accession: NZ_CP049349
• Organism: Escherichia coli strain 3R

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: G6Q95_RS23985
• Protein ID: WP_001351576.1
• Coordinates: 170483..170689 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	170676..170737 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Q95_RS23945	166025..166372	+	348	Protein_162	IS1-like element IS1A family transposase	-
G6Q95_RS23950	166812..167099	-	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	-
G6Q95_RS23955	167096..167347	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
G6Q95_RS23965	168311..169168	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
G6Q95_RS23970	169161..169643	-	483	WP_001273588.1	hypothetical protein	-
G6Q95_RS23975	169636..169710	-	75	WP_001442103.1	RepA leader peptide Tap	-
G6Q95_RS23980	169942..170199	-	258	WP_000083845.1	replication regulatory protein RepA	-
G6Q95_RS23985	170483..170689	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	170676..170737	+	62	NuclAT_1	-	Antitoxin
-	170676..170737	+	62	NuclAT_1	-	Antitoxin
-	170676..170737	+	62	NuclAT_1	-	Antitoxin
-	170676..170737	+	62	NuclAT_1	-	Antitoxin
G6Q95_RS25535	170993..171067	-	75	Protein_170	endonuclease	-
G6Q95_RS23990	171313..171525	-	213	WP_001299730.1	ANR family transcriptional regulator	-
G6Q95_RS23995	171661..172221	-	561	WP_000139315.1	fertility inhibition protein FinO	-
G6Q95_RS24000	172324..173184	-	861	WP_000704513.1	alpha/beta hydrolase	-
G6Q95_RS24005	173243..173989	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat	1..194391	194391

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T151266 WP_001351576.1 NZ_CP049349:c170689-170483 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T151266 NZ_CP065411:3976166-3976441 [Kosakonia radicincitans]
ATGCAGTTAGAATGGACGCGACTCGCTCTTCAGGATGTAGAGAGACTGTCAAAATTCCTGTTTATCCAAAACCCTGAAGC
CGCCCGCAAAGTTGTCATCATGTTGGCTGACGCCCCTGAAATGCTCTTGCTCCACCCACAAATAGGCCCGCCGGATGAAA
GCTGGTATCCGCGCGAAATCCGTTCTTTGTTTGTTGGCAGTTATGAAATGATTTACGAACTGACGGACAGCCATATCATC
ATCCTGCGAATCTGGCATACAAAAGAAGACCGTTAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT151266 NZ_CP049349:170676-170737 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin