Detailed information of TA system TA151258

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 13886..14311
• Replicon: plasmid p3R-1
• Accession: NZ_CP049349
• Organism: Escherichia coli strain 3R

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A0D8W959
• Locus tag: G6Q95_RS23230
• Protein ID: WP_001332444.1
• Coordinates: 13886..14047 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	14100..14311 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Q95_RS23200	9234..9461	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
G6Q95_RS23205	9555..10241	-	687	WP_001825184.1	PAS domain-containing protein	-
G6Q95_RS23210	10432..10815	-	384	WP_000124981.1	relaxosome protein TraM	-
G6Q95_RS23215	11092..11739	+	648	WP_001825185.1	transglycosylase SLT domain-containing protein	-
G6Q95_RS23220	12036..12857	-	822	WP_001234469.1	DUF945 domain-containing protein	-
G6Q95_RS23225	13035..13648	+	614	Protein_19	hypothetical protein	-
G6Q95_RS23230	13886..14047	-	162	WP_001332444.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	14100..14311	-	212	NuclAT_0	-	Antitoxin
-	14100..14311	-	212	NuclAT_0	-	Antitoxin
-	14100..14311	-	212	NuclAT_0	-	Antitoxin
-	14100..14311	-	212	NuclAT_0	-	Antitoxin
G6Q95_RS23235	14322..15041	-	720	WP_001276275.1	plasmid SOS inhibition protein A	-
G6Q95_RS23240	15038..15472	-	435	WP_000845935.1	conjugation system SOS inhibitor PsiB	-
G6Q95_RS23245	15527..17485	-	1959	Protein_23	ParB/RepB/Spo0J family partition protein	-
G6Q95_RS23250	17544..17777	-	234	WP_001209608.1	DUF905 domain-containing protein	-
G6Q95_RS23255	17840..18337	-	498	WP_050427786.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat	1..194391	194391
	-	flank	IS/Tn	-	-	19183..19686	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5976.19 Da
• Isoelectric Point: 8.5101

>T151258 WP_001332444.1 NZ_CP049349:c14047-13886 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T151258 NZ_CP065410:3446434-3446670 [Pseudomonas sp. IAC-BECa141]
ATCGACTACGTTGAACAATACAACTCAAACGCTTCAACGGCTCTGCAGCACAAGGTTGGTGCAGCGACGCAAAGGCTTTC
ATCAATTCCCTATGGTTACCGGTCCGGTCGAGTGCCAGGCACTCGGGAAATGGTGGTCAATCCAAACTATCTGCTGGTCT
ATCGAGTGAACGGGCACATCAAAATACTGACGTTAGTCCACACCCGACGACAATACCCACGAACCTCATCAACCTAA

Antitoxin

Nucleotide

• Length: 212 bp

>AT151258 NZ_CP049349:c14311-14100 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A0D8W959

Antitoxin