Detailed information of TA system TA151254

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4252486..4253088
• Replicon: chromosome
• Accession: NZ_CP049348
• Organism: Escherichia coli strain 3R

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: G6Q95_RS20190
• Protein ID: WP_000897305.1
• Coordinates: 4252486..4252797 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G6Q95_RS20195
• Protein ID: WP_000356397.1
• Coordinates: 4252798..4253088 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Q95_RS20160	4247516..4248301	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
G6Q95_RS20165	4248400..4248999	+	600	WP_001295269.1	glucose-1-phosphatase	-
G6Q95_RS20170	4248993..4249865	+	873	WP_000920762.1	virulence factor BrkB family protein	-
G6Q95_RS20175	4249862..4250299	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
G6Q95_RS20180	4250344..4251285	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
G6Q95_RS20185	4251349..4252257	-	909	WP_001162704.1	alpha/beta hydrolase	-
G6Q95_RS20190	4252486..4252797	+	312	WP_000897305.1	hypothetical protein	Toxin
G6Q95_RS20195	4252798..4253088	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
G6Q95_RS20200	4253674..4253892	+	219	WP_001611167.1	ribbon-helix-helix domain-containing protein	-
G6Q95_RS20205	4254111..4254353	+	243	WP_001086388.1	hypothetical protein	-
G6Q95_RS20210	4254683..4255612	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
G6Q95_RS20215	4255609..4256244	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
G6Q95_RS20220	4256241..4257143	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T151254 WP_000897305.1 NZ_CP049348:4252486-4252797 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T151254 NZ_CP065408:36356-36637 [Diaphorobacter sp. JS3051]
ATGCGGCAGATTGAGCGCACCGGCCAGTTCAAGCGCGACTACAAGCGCGAGGCGAAAGGGCAGCACCGGGCCACGCTCGA
CGCCGACCTGGTGCCCGTCCTGGTCGCGCTCGCCGACGACCAGCCGCTGGAACCTCGGCACCGCGACCACGCGCTGACCG
GGGACTGGAAGGATCACCGGGATTGCCACGTCAAGCCCGACCTGGTGCTGATCTACCAGAAGCCCGACGCCGACACGCTG
CGCCTGGTGCGCCTCGGCTCTCACTCCGAACTCGGCTTGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT151254 WP_000356397.1 NZ_CP049348:4252798-4253088 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT151254 NZ_CP065408:36106-36369 [Diaphorobacter sp. JS3051]
ATGGCTGCAAACCAGCTCGTACAAACGCGCATCGACGGCGCGATCAAGGAAGAAGCGGCGGCCGTCCTGGCCGCGATGGG
CCTTACCGTGTCCGACGCGGTGCGGCTGCTGCTCACGAAGGTGGCCCAGGACAAGGCGCTGCCCTTCGAGCCGCTGATTC
CGAACGCCACCACCATCGAGGCGATGAAGGAAGCCCGCAAGGGCAAGCTGCCGCGCTTCGCCACCGTCAACGATCTGATG
GCCGATCTGCATGCGGCAGATTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7