Detailed information of TA system TA151251

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3410022..3410821
• Replicon: chromosome
• Accession: NZ_CP049348
• Organism: Escherichia coli strain 3R

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F4VJD3
• Locus tag: G6Q95_RS16095
• Protein ID: WP_000347266.1
• Coordinates: 3410357..3410821 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: G6Q95_RS16090
• Protein ID: WP_001307405.1
• Coordinates: 3410022..3410357 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Q95_RS16075	3405807..3406577	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
G6Q95_RS16080	3406593..3407927	-	1335	WP_000599640.1	galactarate/glucarate/glycerate transporter GarP	-
G6Q95_RS16085	3408302..3409873	+	1572	WP_001273741.1	galactarate dehydratase	-
G6Q95_RS16090	3410022..3410357	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
G6Q95_RS16095	3410357..3410821	+	465	WP_000347266.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
G6Q95_RS16100	3410876..3411685	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
G6Q95_RS16105	3411934..3413214	+	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
G6Q95_RS16110	3413237..3413710	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
G6Q95_RS16115	3413721..3414500	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
G6Q95_RS16120	3414490..3415368	+	879	WP_001298758.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
G6Q95_RS16125	3415386..3415820	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3399366..3410821	11455

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17841.18 Da
• Isoelectric Point: 9.4947

>T151251 WP_000347266.1 NZ_CP049348:3410357-3410821 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T151251 NZ_CP065407:c67218-66922 [Diaphorobacter sp. JS3051]
ATGGAGAAACGAACCCCGCATTGCAAGTTGTCAGCCGTCAAAGCCTTGGTAGAGGCTGGCAACGTGCGCACCACGCACGC
GGCGCGTACCGGCGCAAATGAGTTGGGTTTTGCCTTCGCCGACATGCTGGACGTGGTGATGGCACTGACGCCAGCGGATT
TCTACAAGAGCATGACCACCCATGCCGATCACACGGTATGGCAGGACGTGTACCACCCCAGCACGCAGGCGGGTGACGTG
TACCTGAAGCTGACGGTCATTGATGATGTGCTGATCGTGTCCTTTAAGGAGCTATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT151251 WP_001307405.1 NZ_CP049348:3410022-3410357 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT151251 NZ_CP065407:c66919-66518 [Diaphorobacter sp. JS3051]
ATGAAATGTCCTGTTTGCGGCGCGGCGGAACTGATCCATGACACCCGCGACCTACCTTATACCTACAAGGGTGAAACCAC
CGTCATTGCAGCGGTGACGGGCGACTTCTGCCCGGCCTGCGCCGAGTCGGTTTTGGACGCGGCGGAATCAAACCGCGTCA
TGCGCGAAATGCGCGCCTTCTCCAAGCAGGTGAACGCGGCCATCGTTGATCCGGGCTTCATCACGGATGTGCGCAAGAAG
CTTGATCTTGACCAGCGTGAAGCTGCCGAAATCTTCGGCGGTGGCATCAATGCCTTCTCGCGCTACGAGAACGGCAAGAC
CAAGCCGCCGCTGGCCTTGGTCAAGTTGTTCAAGCTGCTAGACCGCCACCCCGACTTGCTCAACGAGGTTAAAGCCGCCT
GA

Structures

Toxin

• AlphaFold DB: A0A836NGD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7