Detailed information of TA system TA151187

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4415909..4416584
• Replicon: chromosome
• Accession: NZ_CP049290
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain 08-175

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: G6324_RS21520
• Protein ID: WP_032619177.1
• Coordinates: 4415909..4416208 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0PXC9
• Locus tag: G6324_RS21525
• Protein ID: WP_015571639.1
• Coordinates: 4416219..4416584 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6324_RS21500 (G6324_21495)	4411041..4411658	-	618	WP_032634496.1	DUF1819 family protein	-
G6324_RS21505 (G6324_21500)	4411822..4412790	-	969	WP_219270774.1	IS5-like element IS903B family transposase	-
G6324_RS21510 (G6324_21505)	4412823..4414478	+	1656	WP_224019229.1	ATP-binding cassette domain-containing protein	-
G6324_RS21515 (G6324_21510)	4414459..4415658	+	1200	WP_017382757.1	HlyD family efflux transporter periplasmic adaptor subunit	-
G6324_RS21520 (G6324_21515)	4415909..4416208	+	300	WP_032619177.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G6324_RS21525 (G6324_21520)	4416219..4416584	+	366	WP_015571639.1	HigA family addiction module antitoxin	Antitoxin
G6324_RS21530 (G6324_21525)	4416611..4417792	-	1182	WP_032619175.1	PLP-dependent aminotransferase family protein	-
G6324_RS21535 (G6324_21530)	4417813..4418700	-	888	WP_032619173.1	LysR family transcriptional regulator	-
G6324_RS21540 (G6324_21535)	4418798..4419400	+	603	WP_017382759.1	short chain dehydrogenase	-
G6324_RS21545 (G6324_21540)	4419397..4420128	-	732	WP_017382760.1	methyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4411822..4412745	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11564.19 Da
• Isoelectric Point: 9.6776

>T151187 WP_032619177.1 NZ_CP049290:4415909-4416208 [Enterobacter hormaechei subsp. steigerwaltii]
MINHSRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T151187 NZ_CP065358:c4812294-4812010 [Kosakonia oryzae]
ATGACCTATGAACTGGTCTTTGATCCGCGGGCATACCGGGAGTGGGAAAAGCTGGGGGAGACAGTAAGAGCGCAATTCAA
AAAGAAACTTGCGGACGTTCTGGTTCAACCACGGATTGAGGCTGCCCGACCGCATGACTTTCCGGATTGCTATAAGATCA
CACTCAGGCCGTCAGGCTACCGGCTGATTTATCAGGTTTGCAATAACGTTGTGACGGTGTTTGTTATCGCTGTGGGAAAG
CGAGAAAAGGCGGCCGCTTACCGGCACGCCCGTAAGCGGTTGTAG

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13705.73 Da
• Isoelectric Point: 9.5936

>AT151187 WP_015571639.1 NZ_CP049290:4416219-4416584 [Enterobacter hormaechei subsp. steigerwaltii]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Nucleotide

• Length: 366 bp

>AT151187 NZ_CP065358:c4812526-4812284 [Kosakonia oryzae]
ATGGCCACGCTAAATGTACGACTGGATGACAATCTCAAGGAAGAGGCCTGGACCGTGCTGGAAAAACTCAATATCACGCC
GACGGAAGCTGTTCGTATGCTGTTTCAGTATGTGGCGGAAAATGGACGTATGCCGATTAAGACCGTGACGGTGAGCGACG
ATGAAGAAGCGCTGTTGAAAATCGTCAGGGAGCGGCTGGCAAATCCGCAGAAAGGCATCAGGGTTAATCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A2J0PXC9