Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3751146..3751806 Replicon chromosome
Accession NZ_CP049290
Organism Enterobacter hormaechei subsp. steigerwaltii strain 08-175

Toxin (Protein)


Gene name higB Uniprot ID A0A2J0Q4X0
Locus tag G6324_RS18365 Protein ID WP_017383312.1
Coordinates 3751453..3751806 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0Q4Z7
Locus tag G6324_RS18360 Protein ID WP_017383313.1
Coordinates 3751146..3751448 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6324_RS18345 (G6324_18345) 3747320..3748645 + 1326 WP_032619371.1 SidA/IucD/PvdA family monooxygenase -
G6324_RS18350 (G6324_18350) 3748672..3750861 + 2190 WP_023303946.1 TonB-dependent siderophore receptor -
G6324_RS18360 (G6324_18360) 3751146..3751448 - 303 WP_017383313.1 XRE family transcriptional regulator Antitoxin
G6324_RS18365 (G6324_18365) 3751453..3751806 - 354 WP_017383312.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
G6324_RS18370 (G6324_18370) 3751997..3752947 - 951 WP_032619370.1 HTH-type transcriptional regulator Cbl -
G6324_RS18375 (G6324_18375) 3753044..3753961 - 918 WP_003859531.1 nitrogen assimilation transcriptional regulator NAC -
G6324_RS18385 (G6324_18385) 3754493..3755425 - 933 WP_023303950.1 L,D-transpeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-106)

Antitoxin

HigA2-like, helix-turn-helix domain

(18-89)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13537.37 Da        Isoelectric Point: 9.9538

>T151186 WP_017383312.1 NZ_CP049290:c3751806-3751453 [Enterobacter hormaechei subsp. steigerwaltii]
VWAIKTTDRFDDWFTSLNDSERASVLAALLVLRERGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREYTRWLNTLKERN

Download         Length: 354 bp

>T151186 NZ_CP065358:4649813-4650130 [Kosakonia oryzae]
ATGCACTTAATCTCAATGAAGGCGATCAAGGAAGCAGTCGATCGCTTTCCTCAGCACAAAACAGCGCTTCTCACGCTGGC
GAAAACGATTGAAAAAGCAGATTGCCCGACACCGAATGATCTTAAAAGGCTTTACCCGACTCTCGATAATTTTAAATATC
TCAGTAAATATTATGTCATTGATATAGCGAACAATAGTCTCAGGATTGTGGCGCTTATCTTTTTCGAAAATCAAAAATTC
TATGTCCGGCATATATTTACGCATAAGGAATATGATCGGTTTACCGAACAACATCGCTCGAAGGGGAAGAAAAAATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11028.85 Da        Isoelectric Point: 6.4727

>AT151186 WP_017383313.1 NZ_CP049290:c3751448-3751146 [Enterobacter hormaechei subsp. steigerwaltii]
MGRTLEQLIADEKPEVVASAQAMATDILLNIHLAELREKVQKTQVDMARALGIKQPTVAVMEKAGRDIKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Download         Length: 303 bp

>AT151186 NZ_CP065358:4650127-4650543 [Kosakonia oryzae]
ATGATCGTCGCTGATGCATTAAAAGCCACCAATGATCTGATTGCTGCGGTTCCCTTATTGGGGGAACACCCGGGCGAAAA
AGATTATCAGGAGGCATTAGAGCTGGTTGAATATCTGCTGGAGAATAATCCCACAACACCGCTACTGGACATTCTCTGCG
CGAAGATTAGCGCTTACGAAAACAGCCAGCCAGACATTATTGCTTTGCGTGAAGAAATGGCAGAAATTCCCCCAGGGATC
GCCGTACTGAGAACATTGATGGATCAGTACCACCTGACCACTTCCGACTTTCAGGATGAAATCGGCAGTAAATCAATGGT
ATCCCGAGTTCTCAACGGTGAGCGCCAACTTAATATCAATCACATCAAGAAATTAGCCGCCCGCTTTGGTGTATCGCCGC
TTTTGTTTATTTCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0Q4X0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0Q4Z7

References