Detailed information of TA system TA151165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3600198..3600925
• Replicon: chromosome
• Accession: NZ_CP049285
• Organism: Shigella boydii strain 602068

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q31WV0
• Locus tag: G5S54_RS18220
• Protein ID: WP_000550185.1
• Coordinates: 3600611..3600925 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G5S54_RS18215
• Protein ID: WP_000560271.1
• Coordinates: 3600198..3600614 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5S54_RS18200	3595558..3596604	+	1047	WP_000695473.1	alpha-glucosidase	-
G5S54_RS18205	3597070..3597909	+	840	Protein_3552	alpha-glucosidase	-
G5S54_RS18210	3598135..3600153	+	2019	WP_000121441.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
G5S54_RS18215	3600198..3600614	-	417	WP_000560271.1	type II toxin-antitoxin system antitoxin HigA	Antitoxin
G5S54_RS18220	3600611..3600925	-	315	WP_000550185.1	type II toxin-antitoxin system toxin HigB	Toxin
G5S54_RS18225	3601210..3602346	-	1137	WP_039080346.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
G5S54_RS18230	3602431..3602934	+	504	WP_001295542.1	M48 family metallopeptidase	-
G5S54_RS18235	3603011..3603694	+	684	WP_001183038.1	vancomycin high temperature exclusion protein	-
G5S54_RS18240	3603773..3604759	+	987	WP_004986115.1	Gfo/Idh/MocA family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12133.19 Da
• Isoelectric Point: 10.0409

>T151165 WP_000550185.1 NZ_CP049285:c3600925-3600611 [Shigella boydii]
MHLITQKALKDAAEKYPQHKTELVALGNMIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T151165 NZ_CP065352:c106847-106317 [Klebsiella pneumoniae]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGG
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15007.47 Da
• Isoelectric Point: 4.4547

>AT151165 WP_000560271.1 NZ_CP049285:c3600614-3600198 [Shigella boydii]
MIAIADILQAGEKLTAVVPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLADLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT151165 NZ_CP065352:c107120-106851 [Klebsiella pneumoniae]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: Q31WV0