Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1702560..1703062 Replicon chromosome
Accession NZ_CP049281
Organism Shigella boydii strain 600657

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag G5S55_RS08775 Protein ID WP_000767829.1
Coordinates 1702808..1703062 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag G5S55_RS08770 Protein ID WP_001259255.1
Coordinates 1702560..1702811 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5S55_RS08745 1697739..1698806 - 1068 WP_000080095.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
G5S55_RS08750 1698806..1699876 - 1071 WP_000108980.1 histidinol-phosphate transaminase -
G5S55_RS08755 1699873..1701177 - 1305 WP_039060143.1 histidinol dehydrogenase -
G5S55_RS08760 1701183..1702082 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
G5S55_RS08765 1702227..1702277 - 51 WP_001364200.1 his operon leader peptide -
G5S55_RS08770 1702560..1702811 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
G5S55_RS08775 1702808..1703062 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
G5S55_RS08780 1703145..1703969 + 825 WP_000754747.1 SDR family oxidoreductase -
G5S55_RS08785 1704015..1704944 + 930 WP_039059232.1 LysR family transcriptional regulator -
G5S55_RS08790 1705159..1705221 + 63 WP_010723108.1 membrane protein YoeI -
G5S55_RS08795 1705211..1706569 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
G5S55_RS08800 1706779..1707708 + 930 WP_024258582.1 YeeE/YedE family protein -
G5S55_RS08805 1707722..1707949 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T151136 WP_000767829.1 NZ_CP049281:1702808-1703062 [Shigella boydii]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T151136 NZ_CP065343:c3393183-3393081 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT151136 WP_001259255.1 NZ_CP049281:1702560-1702811 [Shigella boydii]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT151136 NZ_CP065343:3393074-3393219 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References