Detailed information of TA system TA151133

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1477645..1477870
• Replicon: chromosome
• Accession: NZ_CP049281
• Organism: Shigella boydii strain 600657

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: G5S55_RS07500
• Protein ID: WP_000935258.1
• Coordinates: 1477645..1477857 (-)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1477812..1477870 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5S55_RS07465	1472793..1473949	+	1157	WP_134796775.1	IS3 family transposase	-
G5S55_RS07470	1473993..1474292	-	300	Protein_1440	DUF968 domain-containing protein	-
G5S55_RS07475	1474332..1475870	-	1539	WP_000099170.1	IS66-like element ISEc22 family transposase	-
G5S55_RS07480	1475919..1476266	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
G5S55_RS07485	1476263..1476643	-	381	WP_001333468.1	IS66 family insertion sequence hypothetical protein	-
G5S55_RS07490	1476736..1477197	-	462	Protein_1444	hypothetical protein	-
G5S55_RS07495	1477199..1477477	-	279	WP_000929754.1	hypothetical protein	-
G5S55_RS07500	1477645..1477857	-	213	WP_000935258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	1477812..1477870	+	59	-	-	Antitoxin
G5S55_RS07505	1478452..1478894	-	443	Protein_1447	hypothetical protein	-
G5S55_RS07510	1478938..1479318	+	381	WP_001333468.1	IS66 family insertion sequence hypothetical protein	-
G5S55_RS07515	1479315..1479662	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
G5S55_RS07520	1479711..1481225	+	1515	WP_178930510.1	IS66-like element ISEc22 family transposase	-
G5S55_RS07525	1481246..1481605	+	360	WP_073692993.1	3'-5' exoribonuclease	-
G5S55_RS07530	1481664..1481867	+	204	WP_000096344.1	DUF4224 domain-containing protein	-
G5S55_RS07535	1481867..1482222	+	356	Protein_1453	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	ipaH9.8	1471269..1492110	20841
	-	inside	IScluster/Tn	-	-	1471968..1483423	11455

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7856.42 Da
• Isoelectric Point: 8.7469

>T151133 WP_000935258.1 NZ_CP049281:c1477857-1477645 [Shigella boydii]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 213 bp

>T151133 NZ_CP065343:661204-661737 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT151133 NZ_CP049281:1477812-1477870 [Shigella boydii]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin