Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1806190..1806817 Replicon chromosome
Accession NZ_CP049278
Organism Shigella boydii strain 600690

Toxin (Protein)


Gene name higB Uniprot ID A0A828BKW8
Locus tag G5S56_RS08650 Protein ID WP_024258354.1
Coordinates 1806488..1806817 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q32DW6
Locus tag G5S56_RS08645 Protein ID WP_000147081.1
Coordinates 1806190..1806498 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5S56_RS08610 (1801439) 1801439..1801621 - 183 WP_134795662.1 transposase domain-containing protein -
G5S56_RS08615 (1801637) 1801637..1802793 + 1157 WP_134795588.1 IS3-like element IS600 family transposase -
G5S56_RS08620 (1802885) 1802885..1803472 + 588 Protein_1686 DNA replication protein -
G5S56_RS08625 (1803462) 1803462..1804934 + 1473 WP_139286449.1 DnaB-like helicase C-terminal domain-containing protein -
G5S56_RS08630 (1805011) 1805011..1805451 + 441 WP_000736913.1 recombination protein NinB -
G5S56_RS08635 (1805448) 1805448..1805975 + 528 WP_000153280.1 phage N-6-adenine-methyltransferase -
G5S56_RS08640 (1805972) 1805972..1806148 + 177 WP_078167352.1 NinE family protein -
G5S56_RS08645 (1806190) 1806190..1806498 - 309 WP_000147081.1 helix-turn-helix transcriptional regulator Antitoxin
G5S56_RS08650 (1806488) 1806488..1806817 - 330 WP_024258354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
G5S56_RS08655 (1807024) 1807024..1807410 - 387 Protein_1693 TerL protein -
G5S56_RS08660 (1807413) 1807413..1807970 - 558 WP_078167353.1 terminase small subunit -
G5S56_RS08665 (1808072) 1808072..1808329 - 258 WP_078167354.1 hypothetical protein -
G5S56_RS08670 (1808326) 1808326..1808823 - 498 WP_078167355.1 KilA-N domain-containing protein -
G5S56_RS08675 (1809029) 1809029..1809496 - 468 WP_078167356.1 lysis protein -
G5S56_RS08680 (1809493) 1809493..1809990 - 498 WP_178966610.1 lysozyme RrrD -
G5S56_RS25160 (1809990) 1809990..1810118 - 129 Protein_1699 phage holin -
G5S56_RS08695 (1811383) 1811383..1811472 - 90 Protein_1701 phage holin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1799490..1815641 16151


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-97)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12837.87 Da        Isoelectric Point: 9.9966

>T151112 WP_024258354.1 NZ_CP049278:c1806817-1806488 [Shigella boydii]
MNYTIEYYSEDVRLEVDRLPLSMRARYQHLVERMKIYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIML
HSFVKKTQKTPPAERKKAETRMKEVKHDW

Download         Length: 330 bp

>T151112 NZ_CP065338:c810270-810013 [Klebsiella michiganensis]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGAATCGCGTACATCCGCGATGCATC
AGGCCGCGTCACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAGCTGATTAAGTCCGTCAACGGACAGCCCTACCGCATCAATACCACCGGGCTGAATAACGTCCGCGCA
CAGCTGGACAATCGCTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11185.94 Da        Isoelectric Point: 8.5840

>AT151112 WP_000147081.1 NZ_CP049278:c1806498-1806190 [Shigella boydii]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Download         Length: 309 bp

>AT151112 NZ_CP065338:c810002-809451 [Klebsiella michiganensis]
ATGACAAACCACAGCTTCACTTTTCATGTCGCCTCTGAAGGCGATACCGATGACATTCTCGACGTCGAAACCCGGGCTTT
CGGCTACAGCAAAGAGGCCAGGCTGGTGGCCGATTTGCTCAACGATGAGAGCGCCTGCCCGACGTTATCGCTGCTGGCCA
GGCATAACGGAGAGGCGGTTGGGCATATTTTATTCACCCGGGCGACGTTCAAAGGGGAGCCGGACTCGCCGATGATGCAC
ATCCTTGCTCCCCTGACGGTGGTGCCGGAATACCAGGGCGCGGGCGTTGGCGGAGAGCTTATCCGGCACGGCATTGAGCA
GTTGAAAGCAATGGGAAGCCAGGCGGTATTTGTTCTGGGCCACGCCGCTTACTACCCCAGGCACGGGTTTGAGCCAGGCG
CGGGAGATAAAGGCTATCCGGCGCCTTATCCGATTCCCGCGGCGCATAAAGCCTGCTGGATGCTGCAGCCGATTTCGTCT
CAGCCGCTGGGACGAACGGGCCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828BKW8

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TJC1

References