Detailed information of TA system TA151040

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4655675..4656277
• Replicon: chromosome
• Accession: NZ_CP049204
• Organism: Escherichia coli strain PapM-32-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: G6Z93_RS22040
• Protein ID: WP_000897305.1
• Coordinates: 4655966..4656277 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G6Z93_RS22035
• Protein ID: WP_000356397.1
• Coordinates: 4655675..4655965 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z93_RS22010	4651640..4652542	+	903	WP_032304205.1	formate dehydrogenase O subunit beta	-
G6Z93_RS22015	4652539..4653174	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
G6Z93_RS22020	4653171..4654100	+	930	WP_000027705.1	formate dehydrogenase accessory protein FdhE	-
G6Z93_RS22025	4654413..4654655	-	243	WP_001612096.1	hypothetical protein	-
G6Z93_RS22030	4654873..4655091	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
G6Z93_RS22035	4655675..4655965	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
G6Z93_RS22040	4655966..4656277	-	312	WP_000897305.1	hypothetical protein	Toxin
G6Z93_RS22045	4656506..4657414	+	909	WP_023155176.1	alpha/beta hydrolase	-
G6Z93_RS22050	4657582..4659396	-	1815	WP_164476833.1	hypothetical protein	-
G6Z93_RS22055	4659807..4660748	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
G6Z93_RS22060	4660793..4661230	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T151040 WP_000897305.1 NZ_CP049204:c4656277-4655966 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T151040 NZ_CP065178:1568118-1568546 [Pectobacterium quasiaquaticum]
ATGCTGAAATATCTGCTCGATACCAATATCTGTATTTACACCATCAAGAACAAACCGCAGGAAGTCAGGGACGCTTTCCA
CCGCCATTACGGGCAGTTTGCTATCAGTTCTATCACGTTGATGGAACTGATTTATGGAGCGGAGAAATCGGTAAACCCAG
AAAAGAATCTGGCCGTGATTGAAGGGTTTTCTGCGCGTCTGGAAGTTCAAAACTATGGTTTTGACGCCGCAGTGCATACC
GGCCAAATTCGTGCGGAACTGGCAAAACAAGGAACACCCATCGGCCCGTATGATGCCATGCTAGCGGGTCACGCTCGGTC
AGCAGGTTTGATACTGGTAACGAACAATGTGCGTGAGTTTGAGCGTGTGCCAGGATTGCGCGTAGAAAACTGGGTCGGTA
GACAGGCTGACAATAGCCAGTCTTTGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT151040 WP_000356397.1 NZ_CP049204:c4655965-4655675 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT151040 NZ_CP065178:1567888-1568118 [Pectobacterium quasiaquaticum]
ATGGAAAAAACTACAGTATTTAAAAGCAACCGCAGCCAGGCGGTGCGGCTCCCTAAAGCCGTTGCGCTGCCCGATGATGT
GAAGCAGGTTGATATCGTTGCGATTGGTCGCACACGAATCATTACTCCGGCAGGAGAAAGCTGGGACAGTTGGTTCGAAG
AGGATGGGGTAACACCTGACTTTATGATCACCAGAGAGCAGCCCGATGATCAGATCAGGGAAGATTTCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7