Detailed information of TA system TA151015

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 144710..145131
• Replicon: plasmid pIncFIB
• Accession: NZ_CP049202
• Organism: Escherichia coli strain PapRG-04-4

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: G6Z99_RS26875
• Protein ID: WP_001302184.1
• Coordinates: 144710..144868 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	144933..145131 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z99_RS26840	139776..139991	-	216	WP_000086381.1	conjugal transfer relaxosome protein TraY	-
G6Z99_RS26845	140127..140774	-	648	WP_057698287.1	conjugal transfer transcriptional regulator TraJ	-
G6Z99_RS26850	140965..141348	-	384	WP_001063021.1	relaxosome protein TraM	-
G6Z99_RS26855	141699..142289	+	591	WP_206438904.1	transglycosylase SLT domain-containing protein	-
G6Z99_RS26860	142585..143406	-	822	Protein_162	DUF945 domain-containing protein	-
G6Z99_RS27110	143525..143707	-	183	WP_089607097.1	hypothetical protein	-
G6Z99_RS27115	144155..144472	+	318	WP_001356354.1	hypothetical protein	-
G6Z99_RS26875	144710..144868	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	144933..145131	-	199	NuclAT_0	-	Antitoxin
-	144933..145131	-	199	NuclAT_0	-	Antitoxin
-	144933..145131	-	199	NuclAT_0	-	Antitoxin
-	144933..145131	-	199	NuclAT_0	-	Antitoxin
G6Z99_RS26880	145143..145862	-	720	WP_040072503.1	plasmid SOS inhibition protein A	-
G6Z99_RS26885	145859..146293	-	435	WP_000845926.1	conjugation system SOS inhibitor PsiB	-
G6Z99_RS26890	146348..148306	-	1959	WP_032219150.1	ParB/RepB/Spo0J family partition protein	-
G6Z99_RS26895	148370..148603	-	234	WP_000005988.1	DUF905 family protein	-
G6Z99_RS26900	148666..149205	-	540	WP_032219151.1	single-stranded DNA-binding protein	-
G6Z99_RS27120	149685..150040	+	356	Protein_171	pilus assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pefD / pefC / pefA	1..167766	167766

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T151015 WP_001302184.1 NZ_CP049202:c144868-144710 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T151015 NZ_CP065177:709930-710304 [Pectobacterium quasiaquaticum]
ATGCAACTGTTATCATTGGTTGTGCATGGCCTATTAGTATTGCTCATTCTGCTGGCACCGTGGCCGGACGGCTATGCATG
GCTATGGCTATGCCTGGTCACGATGGTGATGTTCGGTTTTATCCGTAGCCAAAAGAATATCAAATCCCGACAGGGGGAAA
TCGTTCTGCTTAGCGAAACGACCCTGAACTGGCGGCAGCAGGAATGGCAGATCGTTAAACGACCGTGGCTGCTGAAGAAT
GGCGTATTGCTGTCATTGCAAGCGGTTAACGGTAAAGACAAACAACAGCTGTGGCTGGCATCGGACAGCATGGGCGATGA
CGAATGGCGTCACTTGCGTCAAATTCTTTTGCAGCAGAAACACTGGGCAAGATAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT151015 NZ_CP049202:c145131-144933 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGATTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin