Detailed information of TA system TA151009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 4701496..4702001
• Replicon: chromosome
• Accession: NZ_CP049201
• Organism: Escherichia coli strain PapRG-04-4

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: G6Z99_RS22790
• Protein ID: WP_112886714.1
• Coordinates: 4701741..4702001 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A5H6P2W8
• Locus tag: G6Z99_RS22785
• Protein ID: WP_052932205.1
• Coordinates: 4701496..4701741 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z99_RS22765	4696644..4699376	+	2733	WP_164476089.1	DUF927 domain-containing protein	-
G6Z99_RS22770	4699571..4699768	-	198	WP_164476090.1	hypothetical protein	-
G6Z99_RS22775	4699761..4700726	+	966	WP_164476091.1	tyrosine-type recombinase/integrase	-
G6Z99_RS22780	4700755..4701012	-	258	WP_164476092.1	ogr/Delta-like zinc finger family protein	-
G6Z99_RS22785	4701496..4701741	+	246	WP_052932205.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
G6Z99_RS22790	4701741..4702001	+	261	WP_112886714.1	Txe/YoeB family addiction module toxin	Toxin
G6Z99_RS22795	4702321..4703220	-	900	WP_097750698.1	LysR family transcriptional regulator	-
G6Z99_RS22800	4703319..4704041	+	723	WP_164476093.1	SDR family oxidoreductase	-
G6Z99_RS22805	4704207..4704485	+	279	WP_089629567.1	hypothetical protein	-
G6Z99_RS22810	4705186..4706070	-	885	WP_023307638.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4650134..4706070	55936

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10211.89 Da
• Isoelectric Point: 10.1856

>T151009 WP_112886714.1 NZ_CP049201:4701741-4702001 [Escherichia coli]
MKVRISPQAKGDLQYWAENDPKTYNKVMELIKSAKESPFKGIGKPEALKHRKPLWSRRITKEHRLVYYVEQGDLYIVACR
YHYTTI

Nucleotide

• Length: 261 bp

>T151009 NZ_CP065174:c89269-88799 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGTTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACTCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9036.05 Da
• Isoelectric Point: 4.4180

>AT151009 WP_052932205.1 NZ_CP049201:4701496-4701741 [Escherichia coli]
MQHVNYTDARQHLADLMQQANDDRAPILVTRKGAKSVVMIDAEEFASIQETLHLFSNPANAAHIEASLASLERGDYEELD
I

Nucleotide

• Length: 246 bp

>AT151009 NZ_CP065174:c89709-89266 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTTCGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGAGCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A5H6P2W8