Detailed information of TA system TA151008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 4634544..4635085
• Replicon: chromosome
• Accession: NZ_CP049201
• Organism: Escherichia coli strain PapRG-04-4

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: G6Z99_RS22325
• Protein ID: WP_094281256.1
• Coordinates: 4634544..4634822 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: S1EWQ0
• Locus tag: G6Z99_RS22330
• Protein ID: WP_000729704.1
• Coordinates: 4634825..4635085 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z99_RS22310	4632126..4632704	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
G6Z99_RS22315	4632910..4633677	+	768	WP_069913672.1	class II glutamine amidotransferase	-
G6Z99_RS22320	4633648..4634388	-	741	WP_069913671.1	murein L,D-transpeptidase	-
G6Z99_RS22325	4634544..4634822	-	279	WP_094281256.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
G6Z99_RS22330	4634825..4635085	-	261	WP_000729704.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
G6Z99_RS22335	4635295..4636044	+	750	WP_164476262.1	C40 family peptidase	-
G6Z99_RS22340	4636221..4636718	+	498	WP_069913669.1	REP-associated tyrosine transposase RayT	-
G6Z99_RS22345	4637138..4638841	-	1704	Protein_4372	flagellar biosynthesis protein FlhA	-
G6Z99_RS22350	4638822..4639607	+	786	WP_164476077.1	putative lateral flagellar export/assembly protein LafU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (4-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-84)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10867.66 Da
• Isoelectric Point: 9.9107

>T151008 WP_094281256.1 NZ_CP049201:c4634822-4634544 [Escherichia coli]
MFQRDIEYSGQFSKDVKLAQKRHMDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T151008 NZ_CP065173:53790-54272 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9391.77 Da
• Isoelectric Point: 5.0758

>AT151008 WP_000729704.1 NZ_CP049201:c4635085-4634825 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT151008 NZ_CP065173:53536-53802 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6Z6