Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4634544..4635085 Replicon chromosome
Accession NZ_CP049201
Organism Escherichia coli strain PapRG-04-4

Toxin (Protein)


Gene name yafQ Uniprot ID -
Locus tag G6Z99_RS22325 Protein ID WP_094281256.1
Coordinates 4634544..4634822 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag G6Z99_RS22330 Protein ID WP_000729704.1
Coordinates 4634825..4635085 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6Z99_RS22310 4632126..4632704 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
G6Z99_RS22315 4632910..4633677 + 768 WP_069913672.1 class II glutamine amidotransferase -
G6Z99_RS22320 4633648..4634388 - 741 WP_069913671.1 murein L,D-transpeptidase -
G6Z99_RS22325 4634544..4634822 - 279 WP_094281256.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
G6Z99_RS22330 4634825..4635085 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
G6Z99_RS22335 4635295..4636044 + 750 WP_164476262.1 C40 family peptidase -
G6Z99_RS22340 4636221..4636718 + 498 WP_069913669.1 REP-associated tyrosine transposase RayT -
G6Z99_RS22345 4637138..4638841 - 1704 Protein_4372 flagellar biosynthesis protein FlhA -
G6Z99_RS22350 4638822..4639607 + 786 WP_164476077.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10867.66 Da        Isoelectric Point: 9.9107

>T151008 WP_094281256.1 NZ_CP049201:c4634822-4634544 [Escherichia coli]
MFQRDIEYSGQFSKDVKLAQKRHMDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T151008 NZ_CP065173:53790-54272 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT151008 WP_000729704.1 NZ_CP049201:c4635085-4634825 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT151008 NZ_CP065173:53536-53802 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References