Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4709249..4710048 Replicon chromosome
Accession NZ_CP049198
Organism Escherichia coli strain E686

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag G6000_RS22375 Protein ID WP_000347272.1
Coordinates 4709249..4709713 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag G6000_RS22380 Protein ID WP_001307405.1
Coordinates 4709713..4710048 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6000_RS22345 4704250..4704684 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
G6000_RS22350 4704702..4705580 - 879 WP_001298314.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
G6000_RS22355 4705570..4706349 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
G6000_RS22360 4706360..4706833 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
G6000_RS22365 4706856..4708136 - 1281 WP_001521382.1 tagatose-bisphosphate aldolase subunit KbaZ -
G6000_RS22370 4708385..4709194 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
G6000_RS22375 4709249..4709713 - 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
G6000_RS22380 4709713..4710048 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
G6000_RS22385 4710197..4711768 - 1572 WP_001273738.1 galactarate dehydratase -
G6000_RS22390 4712143..4713477 + 1335 WP_089502604.1 galactarate/glucarate/glycerate transporter GarP -
G6000_RS22395 4713493..4714263 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T150979 WP_000347272.1 NZ_CP049198:c4709713-4709249 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

>T150979 NZ_CP065162:4314144-4314362 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT150979 WP_001307405.1 NZ_CP049198:c4710048-4709713 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT150979 NZ_CP065162:4313743-4314117 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References