Detailed information of TA system TA150978

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3859221..3859823
• Replicon: chromosome
• Accession: NZ_CP049198
• Organism: Escherichia coli strain E686

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: G6000_RS18350
• Protein ID: WP_000897305.1
• Coordinates: 3859512..3859823 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G6000_RS18345
• Protein ID: WP_000356395.1
• Coordinates: 3859221..3859511 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6000_RS18315	3854844..3855746	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
G6000_RS18320	3855743..3856378	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
G6000_RS18325	3856375..3857304	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
G6000_RS18330	3857486..3857728	-	243	WP_021523315.1	hypothetical protein	-
G6000_RS18335	3857947..3858165	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
G6000_RS18340	3858584..3858862	-	279	WP_001306650.1	hypothetical protein	-
G6000_RS18345	3859221..3859511	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
G6000_RS18350	3859512..3859823	-	312	WP_000897305.1	hypothetical protein	Toxin
G6000_RS18355	3860052..3860960	+	909	WP_001306651.1	alpha/beta hydrolase	-
G6000_RS18360	3861129..3862943	-	1815	WP_020233236.1	hypothetical protein	-
G6000_RS18365	3863359..3864300	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
G6000_RS18370	3864345..3864782	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T150978 WP_000897305.1 NZ_CP049198:c3859823-3859512 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T150978 NZ_CP065162:c4142461-4142144 [Klebsiella variicola]
ATATTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGCCAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTATGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT150978 WP_000356395.1 NZ_CP049198:c3859511-3859221 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT150978 NZ_CP065162:c4142151-4141864 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7