Detailed information of TA system TA150971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2907184..2907878
• Replicon: chromosome
• Accession: NZ_CP049198
• Organism: Escherichia coli strain E686

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: G6000_RS13855
• Protein ID: WP_001521903.1
• Coordinates: 2907184..2907582 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: G6000_RS13860
• Protein ID: WP_000554758.1
• Coordinates: 2907585..2907878 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6000_RS13825	2902354..2903598	-	1245	WP_000189541.1	esterase FrsA	-
-	2903014..2903094	-	81	NuclAT_11	-	-
-	2903014..2903094	-	81	NuclAT_11	-	-
-	2903014..2903094	-	81	NuclAT_11	-	-
-	2903014..2903094	-	81	NuclAT_11	-	-
G6000_RS13830	2903690..2904148	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
G6000_RS13835	2904409..2905866	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
G6000_RS13840	2905923..2906275	-	353	Protein_2709	peptide chain release factor H	-
G6000_RS13845	2906271..2906477	-	207	Protein_2710	RtcB family protein	-
G6000_RS13850	2906722..2907174	-	453	WP_023144376.1	GNAT family N-acetyltransferase	-
G6000_RS13855	2907184..2907582	-	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
G6000_RS13860	2907585..2907878	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
G6000_RS13865	2907930..2908985	-	1056	WP_001226177.1	DNA polymerase IV	-
G6000_RS13870	2909056..2909841	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
G6000_RS13875	2909813..2911519	+	1707	Protein_2716	flagellar biosynthesis protein FlhA	-
G6000_RS13880	2911603..2911956	+	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
G6000_RS13885	2912013..2912762	-	750	WP_016233951.1	C40 family peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2897121..2907878	10757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T150971 WP_001521903.1 NZ_CP049198:c2907582-2907184 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T150971 NZ_CP065162:320480-320848 [Klebsiella variicola]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGACGTTGCCGG
TATGCCCGACCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCACCTGCTGGTGATTTCTCGCGGCCATATTTTCAATGATGGAAATAAGCGTACGGCACTGTTT
ATTACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT150971 WP_000554758.1 NZ_CP049198:c2907878-2907585 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT150971 NZ_CP065162:320262-320483 [Klebsiella variicola]
ATGAGAACGGTTAACTATAGCGAAGCCCGACAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCGGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATTATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3