150955

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 860163..860665 Replicon chromosome
Accession NZ_CP049198
Organism Escherichia coli strain E686

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag G6000_RS03995 Protein ID WP_000767829.1
Coordinates 860411..860665 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag G6000_RS03990 Protein ID WP_001259255.1
Coordinates 860163..860414 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6000_RS03965 855341..856408 - 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
G6000_RS03970 856408..857478 - 1071 WP_000108937.1 histidinol-phosphate transaminase -
G6000_RS03975 857475..858779 - 1305 WP_021523144.1 histidinol dehydrogenase -
G6000_RS03980 858785..859684 - 900 WP_021523143.1 ATP phosphoribosyltransferase -
G6000_RS03985 859830..859880 - 51 WP_001364200.1 his operon leader peptide -
G6000_RS03990 860163..860414 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
G6000_RS03995 860411..860665 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
G6000_RS04000 860748..861572 + 825 WP_000754758.1 SDR family oxidoreductase -
G6000_RS04005 861618..862547 + 930 WP_023154044.1 LysR family transcriptional regulator -
G6000_RS04010 862762..862824 + 63 WP_010723108.1 membrane protein YoeI -
G6000_RS04015 862814..864172 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
G6000_RS04020 864351..865409 + 1059 WP_000492324.1 transport protein YeeE -
G6000_RS04025 865423..865650 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T150955 WP_000767829.1 NZ_CP049198:860411-860665 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T150955 NZ_CP065152:c4333332-4333078 [Escherichia coli O150:H6]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT150955 WP_001259255.1 NZ_CP049198:860163-860414 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT150955 NZ_CP065152:c4333580-4333329 [Escherichia coli O150:H6]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References