Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 860163..860665 Replicon chromosome
Accession NZ_CP049198
Organism Escherichia coli strain E686

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag G6000_RS03995 Protein ID WP_000767829.1
Coordinates 860411..860665 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag G6000_RS03990 Protein ID WP_001259255.1
Coordinates 860163..860414 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6000_RS03965 855341..856408 - 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
G6000_RS03970 856408..857478 - 1071 WP_000108937.1 histidinol-phosphate transaminase -
G6000_RS03975 857475..858779 - 1305 WP_021523144.1 histidinol dehydrogenase -
G6000_RS03980 858785..859684 - 900 WP_021523143.1 ATP phosphoribosyltransferase -
G6000_RS03985 859830..859880 - 51 WP_001364200.1 his operon leader peptide -
G6000_RS03990 860163..860414 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
G6000_RS03995 860411..860665 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
G6000_RS04000 860748..861572 + 825 WP_000754758.1 SDR family oxidoreductase -
G6000_RS04005 861618..862547 + 930 WP_023154044.1 LysR family transcriptional regulator -
G6000_RS04010 862762..862824 + 63 WP_010723108.1 membrane protein YoeI -
G6000_RS04015 862814..864172 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
G6000_RS04020 864351..865409 + 1059 WP_000492324.1 transport protein YeeE -
G6000_RS04025 865423..865650 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T150955 WP_000767829.1 NZ_CP049198:860411-860665 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T150955 NZ_CP065152:c4333332-4333078 [Escherichia coli O150:H6]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT150955 WP_001259255.1 NZ_CP049198:860163-860414 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT150955 NZ_CP065152:c4333580-4333329 [Escherichia coli O150:H6]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References