Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 5010514..5011313 Replicon chromosome
Accession NZ_CP049197
Organism Escherichia coli strain E597

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag G6Z98_RS23750 Protein ID WP_000347272.1
Coordinates 5010514..5010978 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag G6Z98_RS23755 Protein ID WP_001307405.1
Coordinates 5010978..5011313 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6Z98_RS23720 5005515..5005949 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
G6Z98_RS23725 5005967..5006845 - 879 WP_001298314.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
G6Z98_RS23730 5006835..5007614 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
G6Z98_RS23735 5007625..5008098 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
G6Z98_RS23740 5008121..5009401 - 1281 WP_001521382.1 tagatose-bisphosphate aldolase subunit KbaZ -
G6Z98_RS23745 5009650..5010459 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
G6Z98_RS23750 5010514..5010978 - 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
G6Z98_RS23755 5010978..5011313 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
G6Z98_RS23760 5011462..5013033 - 1572 WP_001273738.1 galactarate dehydratase -
G6Z98_RS23765 5013408..5014742 + 1335 WP_089502604.1 galactarate/glucarate/glycerate transporter GarP -
G6Z98_RS23770 5014758..5015528 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5010514..5021965 11451


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T150952 WP_000347272.1 NZ_CP049197:c5010978-5010514 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

>T150952 NZ_CP065152:c3755574-3755296 [Escherichia coli O150:H6]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT150952 WP_001307405.1 NZ_CP049197:c5011313-5010978 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT150952 NZ_CP065152:c3755296-3755012 [Escherichia coli O150:H6]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References