Detailed information of TA system TA150944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3204941..3205635
• Replicon: chromosome
• Accession: NZ_CP049197
• Organism: Escherichia coli strain E597

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: G6Z98_RS15215
• Protein ID: WP_001521903.1
• Coordinates: 3204941..3205339 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: G6Z98_RS15220
• Protein ID: WP_000554758.1
• Coordinates: 3205342..3205635 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z98_RS15185	3200111..3201355	-	1245	WP_000189541.1	esterase FrsA	-
-	3200771..3200851	-	81	NuclAT_11	-	-
-	3200771..3200851	-	81	NuclAT_11	-	-
-	3200771..3200851	-	81	NuclAT_11	-	-
-	3200771..3200851	-	81	NuclAT_11	-	-
G6Z98_RS15190	3201447..3201905	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
G6Z98_RS15195	3202166..3203623	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
G6Z98_RS15200	3203680..3204032	-	353	Protein_2971	peptide chain release factor H	-
G6Z98_RS15205	3204028..3204234	-	207	Protein_2972	RtcB family protein	-
G6Z98_RS15210	3204479..3204931	-	453	WP_023144376.1	GNAT family N-acetyltransferase	-
G6Z98_RS15215	3204941..3205339	-	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
G6Z98_RS15220	3205342..3205635	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
G6Z98_RS15225	3205687..3206742	-	1056	WP_001226177.1	DNA polymerase IV	-
G6Z98_RS15230	3206813..3207598	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
G6Z98_RS15235	3207570..3209282	+	1713	Protein_2978	flagellar biosynthesis protein FlhA	-
G6Z98_RS15240	3209361..3209714	+	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
G6Z98_RS15245	3209771..3210520	-	750	WP_016233951.1	C40 family peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3194878..3205635	10757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T150944 WP_001521903.1 NZ_CP049197:c3205339-3204941 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T150944 NZ_CP065152:1863229-1863402 [Escherichia coli O150:H6]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT150944 WP_000554758.1 NZ_CP049197:c3205635-3205342 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT150944 NZ_CP065152:1862905-1863201 [Escherichia coli O150:H6]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3